GM08011
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/MOUSE SOMATIC CELL HYBRIDS
Repository
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NIGMS Human Genetic Cell Repository
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Subcollection |
Hybrids |
Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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No intact human chromosomes were observed with G11 staining
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Remarks
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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Remarks |
Line NBR4-RJK835; produced by fusing NBmouse neuroblastoma line, HPRT- (Dr K Prasad) with HeLa (S3 clone of Puck) minicells & selection in HAT medium; line has elev HPRT activity & approx 50 copies of HPRT gene |
Melton DW, Cell fusion-induced mouse neuroblastomas HPRT revertants with variant enzyme and elevated HPRT protein levels. Somatic Cell Genet7:331-44 1981 |
PubMed ID: 7027497 |
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Melton DW, Konecki DS, Ledbetter DH, Hejtmancik JF, Caskey CT, In vitro translation of hypoxanthine/guanine phosphoribosyltransferase mRNA: characterization of a mouse neuroblastoma cell line that has elevated levels of hypoxanthine/guanine phosphoribosyltransferase protein. Proc Natl Acad Sci U S A78:6977-80 1981 |
PubMed ID: 6947270 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
8% |
Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Aminopterin 4 x 10-7M |
Supplement |
Hypoxanthine 1 x 10-4M |
Supplement |
Thymidine 1.6 x 10-5M |
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