GM10501
Somatic cell hybrid
Description:
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME X REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Chinese hamster/human hybrid retaining human der(X)t(X;17) (97%), & #8 (93%), 12 (93%), 14 (30%), 18 (4%) & 21 (70%)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for Xp22.32 and Yp11.3, MIC2, and gave a positive result with a probe for Xq28, DXS15. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp11.21, ALAS2, Xq12, DXS453, Xq13.3, PGK1, Xq21.3, DXS3, Xq26, HPRT, and Xq28, G6PD, and gave negative results with Xp11.3, DXS1003, and Xp22.32, STS. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 12p13.2, PRB1, and 12q14q24.1, D12S7. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17q25, P4HB, and gave a positive result with a probe for 17p13.1, MYH2. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 17p13.1, TP53, and gave negative results with primers for 17q21.3-q23, MPO, and 17q25-qter, D17S784. . |
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| Remarks |
Line 88H-5; produced by PEG fusion of human fibroblasts, GM07151, 46,X,t(X;17) (p11.21;p11.1), with HPRT-deficient Chinese hamster cell line RJK88; selected in HAT medium; retains der(X)t(X;17) in 97% of cells & other human chromos |
| Moran JL, Johnston SH, Rauskolb C, Bhalerao J, Bowcock AM, Vogt TF, Genomic structure, mapping, and expression analysis of the mammalian Lunatic,
Manic, and Radical fringe genes. Mamm Genome10(6):535-41 1999 |
| PubMed ID: 10341080 |
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| Plummer SJ, Simmons JA, Adams L, Casey G, Mapping of 228 ESTs and 26 genes into an integrated physical and genetic map of human chromosome 17. Genomics45:140-6 1997 |
| PubMed ID: 9339370 |
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| Boycott KM, Halley GR, Schlessinger D, Bech-Hansen NT, A 2-megabase physical contig incorporating 43 DNA markers on the human X chromosome at p11.23-p11.22 from ZNF21 to DXS255. Genomics33:488-97 1996 |
| PubMed ID: 8661008 |
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| Kusuda J, Hidari N, Hirai M, Hashimoto K, Sequence analysis of the cDNA for the human casein kinase I delta (CSNK1D) gene and its chromosomal localization. Genomics32:140-3 1996 |
| PubMed ID: 8786104 |
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| Swanson DA, Freund CL, Ploder L, McInnes RR, Valle D, A ubiquitin C-terminal hydrolase gene on the proximal short arm of the X chromosome: implications for X-linked retinal disorders Human molecular genetics5:533-8 1996 |
| PubMed ID: 8845848 |
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| Bloch KD, Wolfram JR, Brown DM, Roberts JD Jr, Zapol DG, Lepore JJ, Filippov G, Thomas JE, Jacob HJ, Bloch DB, Three members of the nitric oxide synthase II gene family (NOS2A, NOS2B, and NOS2C) colocalize to human chromosome 17. Genomics27:526-30 1995 |
| PubMed ID: 7558036 |
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| Flejter WL, Bennett-Baker P, Barcroft CL, Kiousis S, Chamberlain JS, Region-specific cosmids and STRPs identified by chromosome microdissection and FISH. Genomics25:413-20 1995 |
| PubMed ID: 7789975 |
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| Chang DY, Nelson B, Bilyeu T, Hsu K, Darlington GJ, Maraia RJ, A human Alu RNA-binding protein whose expression is associated with accumulation of small cytoplasmic Alu RNA. Mol Cell Biol14:3949-59 1994 |
| PubMed ID: 8196634 |
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| Driscoll CT, Darlington GJ, Maraia RJ, The conserved 7SK snRNA gene localizes to human chromosome 6 by homolog
exclusion probing of somatic cell hybrid RNA. Nucleic Acids Res22(5):722-5 1994 |
| PubMed ID: 8139910 |
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| Umbricht CB, Griffin CA, Hawkins AL, Grzeschik KH, O'Connell P, Leach R, Green ED, Kelly TJ, High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3). Genomics20:249-57 1994 |
| PubMed ID: 8020972 |
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| Corcos IA, Lafreniere RG, Begy CR, Loch-Caruso R, Willard HF, Glover TW, Refined localization of human connexin32 gene locus, GJB1, to Xq13.1. Genomics13:479-80 1992 |
| PubMed ID: 1319395 |
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| Gorski JL, Boehnke M, Reyner EL, Burright EN, A radiation hybrid map of the proximal short arm of the human X chromosome spanning incontinentia pigmenti 1 (IP1) translocation breakpoints. Genomics14:657-65 1992 |
| PubMed ID: 1427892 |
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| Gorski JL, Burright EN, Reyner EL, Goodfellow PN, Burgess DL, Isolation of DNA markers from a region between incontinentia pigmenti 1 (IP1) X-chromosomal translocation breakpoints by a comparative PCR analysis of a radiation hybrid subclone mapping panel. Genomics14:649-56 1992 |
| PubMed ID: 1427891 |
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| Arenstorf HP, Kandpal RP, Baskaran N, Parimoo S, Tanaka Y, Kitajima S, Yasukochi Y, Weissman SM, Construction and characterization of a NotI-BsuE linking library from the human X chromosome. Genomics11:115-23 1991 |
| PubMed ID: 1765370 |
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| van Tuinen P, Rich DC, Summers KM, Ledbetter DH, Regional mapping panel for human chromosome 17: application to neurofibromatosis type 1. Genomics1:374-81 1987 |
| PubMed ID: 3130306 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Percent O2 |
AMBIENT |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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