GM10664
Somatic cell hybrid
Description:
CHROMOSOME 15 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 10 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Human/Chinese hamster hybrid retaining a human der(15)t(X;15) in 100% of cells & a #22 in 100% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for Xp22.32 and Yp11.3, MIC2, and gave a positive result with a probe for Xq28, DXS15. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for Xp11.3, DXS1003, Xp22.32, STS, Xq12, DXS453, Xq13.3, PGK1, and Xq21.3, DXS3, and gave positive results with primers for Xq26, HPRT, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 5q33q35, PDGFRB. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 6q24q27, ESR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 15q25qter, IGF1R. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 15q21-q22.2, B2M, 15q11-q12, D15S11, 15q11.2q12, GABRB3, and 15q23, D15S1485A. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17p13.1, MYH2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 22q13, D22S45, and 22q12.3q13.1, PDGFB. |
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| Remarks |
Line 94-3; produced by the PEG fusion of lymphoblasts from a pt with a t(X;15) (q25 or q26;q25) & the HPRT-def Chinese hamster cell line RJK88; selected in HZ medium; retains the human der(15) & human chromosome #22 in 100% of cells |
| Swanson DA, Freund CL, Ploder L, McInnes RR, Valle D, A ubiquitin C-terminal hydrolase gene on the proximal short arm of the X chromosome: implications for X-linked retinal disorders Human molecular genetics5:533-8 1996 |
| PubMed ID: 8845848 |
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| Argeson AC, Druck T, Veronese ML, Knopf JL, Buchberg AM, Huebner K, Siracusa LD, Phospholipase C gamma-2 (Plcg2) and phospholipase C gamma-1 (Plcg1) map to distinct regions in the human and mouse genomes. Genomics25:29-35 1995 |
| PubMed ID: 7774933 |
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| Ellis NA, Groden J, Ye TZ, Straughen J, Lennon DJ, Ciocci S, Proytcheva M, German J, The Bloom's syndrome gene product is homologous to RecQ helicases. Cell83:655-66 1995 |
| PubMed ID: 7585968 |
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| Driscoll CT, Darlington GJ, Maraia RJ, The conserved 7SK snRNA gene localizes to human chromosome 6 by homolog
exclusion probing of somatic cell hybrid RNA. Nucleic Acids Res22(5):722-5 1994 |
| PubMed ID: 8139910 |
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| Oaks MK, Hanson JP Jr, O'Malley DP, Molecular cytogenetic mapping of the human melanoma antigen (MAGE) gene family to chromosome region Xq27-qter: implications for MAGE immunotherapy. Cancer Res54:1627-9 1994 |
| PubMed ID: 8137270 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Azaserine 4.4 x 10-5M |
| Supplement |
Hypoxanthine 1 x 10-4M |
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