GM11023
Somatic cell hybrid
Description:
CHROMOSOME X REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/mouse hybrid retaining human der (X)t(X;4), #3 through 8, 10, 11, & 14 through 22
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp11.3, DXS1003, Xq12, DXS453, Xq13.3, PGK1, Xq21.3, DXS3, Xq26, HPRT, and Xq28, G6PD, and gave a negative result with a primer for Xp22.32, STS. |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for Yq11, DYS227. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 2p13, TGFA, 2p23, POMC, 2q13, IL1A, and 2q37, ALPP. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 4p15.3, QDPR, and 4q31-q33, 4S1626. |
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| Remarks |
Line IC-C3; produced by fusing human lymphoblasts, GM11025, 46,XX,t(X;4)(p21; q35), with HPRT-def, ouabain-resistant mouse RAG cells; selected in HAT medium; retains hum der(X)t(X;4) in 96% of cells & other human chromosomes including #4 |
| Harun RB, Smith KK, Leek JP, Markham AF, Norris A, Morrison JF, Characterization of human SHC p66 cDNA and its processed pseudogene mapping to Xq12-q13.1. Genomics42:349-52 1997 |
| PubMed ID: 9192859 |
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| Mills KA, Buetow KH, Xu Y, Ritty TM, Mathews KD, Bodrug SE, Wijmenga C, Balazs I, Murray JC, Genetic and physical mapping on chromosome 4 narrows the localization of the gene for facioscapulohumeral muscular dystrophy (FSHD). Am J Hum Genet51:432-9 1992 |
| PubMed ID: 1642243 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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