GM11130
Somatic cell hybrid
Description:
CHROMOSOME 21 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 1 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/mouse hybrid retaining human chromosomes #2 (66%), 18 (56%), der(21 (60%), & X (90%)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 1p36, PND, and gave a negative result with a probe for 1q32, REN. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 1p13, NGFB, and 1q32, REN, and gave a positive result with a primer for 1p36.2, TNFR2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 2p25, D2S1, and 2q37, D2S3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 3p25, RAF1, and 3q26.2qter, TFRC. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 21q22.1q22.2, D21S17. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 21q21.2, APP, 21q21.2, AMYLOID, and 21q22.3, ITGB2, and gave positive results with primers for 21p11.2, D21S1278 and 21q11, D21S120. |
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| Remarks |
Line AHVI-17; produced by fusing fibroblasts, GM01881, 46,XY,t(1;21)(p31;q22) with HPRT-deficient mouse line A9; retains human der(21)t(1;21)(p22;q21.2) & human chromosomes #2, 18, & X in 60%, 66%, 56%, & 9O% of cells respectively |
| Marshall LJ, Moore AC, Ohki M, Kitabayashi I, Patterson D, Ornelles DA, RUNX1 permits E4orf6-directed nuclear localization of the adenovirus E1B-55K protein and associates with centers of viral DNA and RNA synthesis Journal of virology82:6395-408 2008 |
| PubMed ID: 18417565 |
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| Chastain-Moore AM, Roberts T, Trott DA, Newbold RF, Ornelles DA, An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression. J Virol77(14):8087-98 2003 |
| PubMed ID: 12829847 |
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| White RA, Adkison LR, Dowler LL, Ray RB, Chromosomal localization of the human gene encoding c-myc promoter- binding protein (MPB1) to chromosome 1p35-pter. Genomics39:406-8 1997 |
| PubMed ID: 9119380 |
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| Hirai M, Kusuda J, Hashimoto K, Assignment of human ADP ribosylation factor (ARF) genes ARF1 and ARF3 to chromosomes 1q42 and 12q13, respectively. Genomics34(2):263-5 1996 |
| PubMed ID: 8661066 |
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| Argeson AC, Druck T, Veronese ML, Knopf JL, Buchberg AM, Huebner K, Siracusa LD, Phospholipase C gamma-2 (Plcg2) and phospholipase C gamma-1 (Plcg1) map to distinct regions in the human and mouse genomes. Genomics25:29-35 1995 |
| PubMed ID: 7774933 |
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| Dorig RE, Marcil A, Chopra A, Richardson CD, The human CD46 molecule is a receptor for measles virus (Edmonston strain). Cell75:295-305 1993 |
| PubMed ID: 8402913 |
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| Virtaneva KI, Angelisova P, Baumruker T, Horejsi V, Nevanlinna H, Schroder J, The genes for CD37, CD53, and R2, all members of a novel gene family, are located on different chromosomes. Immunogenetics37:461-5 1993 |
| PubMed ID: 8436422 |
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| Korenberg JR, Croyle ML, Cox DR, Isolation and regional mapping of DNA sequences unique to human chromosome 21. Am J Hum Genet41:963-78 1987 |
| PubMed ID: 2891299 |
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| Cox DR, Epstein CJ, Comparative gene mapping of human chromosome 21 and mouse chromosome 16. Ann N Y Acad Sci450:169-177 1985 |
| PubMed ID: 3160288 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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