GM11172
Somatic cell hybrid
Description:
CHROMOSOME X REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 2 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Karyotypic analysis and In situ hybridization
|
|
ISCN
|
Human/Chinese hamster hybrid retaining human der(2)t(X;2)(q21.3;q22) (81%) & human #11 (54%), #19 (46%), & #22 (96%)
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xq21.3, DXS3, Xq23, DXS1059, Xq26, HPRT, and Xq28, G6PD, and gave negative results with primers for Xp11.21, ALAS2, Xp11.3, DXS1003, Xp22.32, STS, Xq12, DXS453, and Xq13.3, PGK1. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 2q37, ALPP, and gave positive results with primers for 2p13, TGFA, 2p23, POMC, and 2q13, IL1A. . |
| |
| Remarks |
Line Ben3B; produced by the fusion of human lymphoblasts, t(X;2)(q21.3;q22), with HPRT-def Chinese hamster RJK88 cells; grown in medium with hypoxanthine & azaserine; retains the human der(2)t (X;2) (81% of cells) & other hum chromos |
| Gleeson JG, Allen KM, Fox JW, Lamperti ED, Berkovic S, Scheffer I, Cooper EC, Dobyns WB, Minnerath SR, Ross ME, Walsh CA, Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein. Cell92:63-72 1998 |
| PubMed ID: 9489700 |
| |
| Swanson DA, Freund CL, Ploder L, McInnes RR, Valle D, A ubiquitin C-terminal hydrolase gene on the proximal short arm of the X chromosome: implications for X-linked retinal disorders Human molecular genetics5:533-8 1996 |
| PubMed ID: 8845848 |
| |
| Wedemeyer N, Lengeling A, Ronsiek M, Korthaus D, Baer K, Wuttke M, Jockusch H, YAC contigs of the Rab1 and wobbler (wr) spinal muscular atrophy gene region on proximal mouse chromosome 11 and of the homologous region on human chromosome 2p. Genomics32:447-54 1996 |
| PubMed ID: 8838809 |
| |
| Oaks MK, Hanson JP Jr, O'Malley DP, Molecular cytogenetic mapping of the human melanoma antigen (MAGE) gene family to chromosome region Xq27-qter: implications for MAGE immunotherapy. Cancer Res54:1627-9 1994 |
| PubMed ID: 8137270 |
| |
| Arenstorf HP, Kandpal RP, Baskaran N, Parimoo S, Tanaka Y, Kitajima S, Yasukochi Y, Weissman SM, Construction and characterization of a NotI-BsuE linking library from the human X chromosome. Genomics11:115-23 1991 |
| PubMed ID: 1765370 |
| |
| Luty JA, Guo Z, Willard HF, Ledbetter DH, Ledbetter S, Litt M, Five polymorphic microsatellite VNTRs on the human X chromosome. Am J Hum Genet46:776-83 1990 |
| PubMed ID: 2316523 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Azaserine 4.4 x 10-5M |
| Supplement |
Hypoxanthine 1 x 10-4M |
|
|