GM11173
Somatic cell hybrid
Description:
CHROMOSOME 1 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME X REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/Chinese hamster hybrid retaining human der(X)t(X;1) (92%), #11 (92%), #13 (80%), #18 (88%), & #21 (52%)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for Xp22.32, STS, and gave positive results with primers for Xp11.3, DXS1003, Xq12, DXS453, Xq13.3, PGK1, Xq21.3, DXS3, Xq26, HPRT, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 1p36.2, TNFR2, and 1p13, NGFB, and gave negative results with primers for 1q32, REN, and 1q44, SV-1_2004. |
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| Remarks |
Line AA-H; produced by fusion of human lymphos from female with DMD, t(X;1) (p21.2;p12), with HPRT-def Chinese hamster RJK88 cells; grown in medium with hypoxanthine & azaserine; retains human der(X) (92% of cells) & other hum chromo |
| Swanson DA, Freund CL, Ploder L, McInnes RR, Valle D, A ubiquitin C-terminal hydrolase gene on the proximal short arm of the X chromosome: implications for X-linked retinal disorders Human molecular genetics5:533-8 1996 |
| PubMed ID: 8845848 |
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| Virtaneva KI, Angelisova P, Baumruker T, Horejsi V, Nevanlinna H, Schroder J, The genes for CD37, CD53, and R2, all members of a novel gene family, are located on different chromosomes. Immunogenetics37:461-5 1993 |
| PubMed ID: 8436422 |
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| Arenstorf HP, Kandpal RP, Baskaran N, Parimoo S, Tanaka Y, Kitajima S, Yasukochi Y, Weissman SM, Construction and characterization of a NotI-BsuE linking library from the human X chromosome. Genomics11:115-23 1991 |
| PubMed ID: 1765370 |
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| Luty JA, Guo Z, Willard HF, Ledbetter DH, Ledbetter S, Litt M, Five polymorphic microsatellite VNTRs on the human X chromosome. Am J Hum Genet46:776-83 1990 |
| PubMed ID: 2316523 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Azaserine 4.4 x 10-5M |
| Supplement |
Hypoxanthine 1 x 10-4M |
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