GM11222
Somatic cell hybrid
Description:
CHROMOSOME 22 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 11 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/Chinese hamster hybrid retaining human der(11)t(11;22) (92% of cells) & #3, 4, 5, 6, 8, 9, 14, 15, 16, & 18
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 11p13, CAT, and 11q23.3, ETS1. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 11p13, CAT, and 11q21-q22, CLG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 22q13.31, KI-108, and 22q13.31-qter, DIA1, and gave negative results with primers for 22q11.2, IGLC2, and 22q12.1q13.1, D22S300. |
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| Remarks |
Line A3EW2-3B; produced by the fusion of IARC-EW2 cells, der(11)t(11;22)(q24; q12), with TK-deficient Chinese hamster a3 cells; grown in HAT medium; retains human der(11)t(11;22) in 92% of cells and other human chromosomes |
| Prawitt D, Enklaar T, Klemm G, Gartner B, Spangenberg C, Winterpacht A,
Higgins M, Pelletier J, Zabel B, Identification and characterization of MTR1, a novel gene with homology to
melastatin (MLSN1) and the trp gene family located in the BWS-WT2 critical
region on chromosome 11p15.5 and showing allele-specific expression. Curr Biol9(2):203-16 2000 |
| PubMed ID: 10607831 |
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| Leek J, Lench N, Maraj B, Bailey A, Carr IM, Andersen S, Cross J, Whelan P, MacLennan KA, Meredith DM, Prostate-specific membrane antigen: evidence for the existence of a second related human gene British journal of cancer72:583-8 1995 |
| PubMed ID: 7669565 |
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| Golden WL, von Kap-Herr C, Kurth B, Wright RM, Flickinger CJ, Eddy R, Shows T, Herr JC, Refinement of the localization of the gene for human intraacrosomal protein SP-10 (ACRV1) to the junction of bands q23-->q24 of chromosome 11 by nonisotopic in situ hybridization. Genomics18:446-9 1993 |
| PubMed ID: 8288254 |
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| Morris C, Courtay C, Geurts van Kessel A, ten Hoeve J, Heisterkamp N, Groffen J, Localization of a gamma-glutamyl-transferase-related gene family on chromosome 22. Hum Genet91:31-6 1993 |
| PubMed ID: 8095916 |
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| Herzog R, Lutz S, Blin N, Marasa JC, Blinder MA, Tollefsen DM, Complete nucleotide sequence of the gene for human heparin cofactor II and mapping to chromosomal band 22q11. Biochemistry30:1350-1357 1991 |
| PubMed ID: 1671335 |
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| van Kessel AG, Turc-Carel C, de Klein A, Grosveld G, Lenoir G, Bootsma D, Translocation of oncogene c-sis from chromosome 22 to chromosome 11 in a Ewing sarcoma-derived cell line. Mol Cell Biol5:427-9 1985 |
| PubMed ID: 2983195 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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