GM11434
Somatic cell hybrid
Description:
CHROMOSOME 5 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Chinese hamster somatic cell hybrid retaining a human del(5) in 100% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for Yq11, DYS227. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 2p25, D2S1, and 2q37, D2S3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 3p25, RAF1, and 3q26.2qter, TFRC. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 5pterp15.3, D5S10, and 5q33q35, PDGFRB. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 5p14-p12, C9, 5p15.3-p15.1, D5S208, 5q32, D5S372, and 5q34-q35, GABRA1, and gave negative results with primers for 5q11.2-q13.1, D5S351, and 5q13.3-q14, HMGCR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 21q11, D21S26, and 21q22.1q22.2, D21S17. |
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| Remarks |
Line HHW1064; human/Chinese hamster hybrid retaining a human del(5)(5qter>5q 13.3::5q11.2>5pter) in 100% of cells; in 44% of cells the del(5) chromosomes were broken at the centromere; grown in culture medium with 0.2mM proline at 39C |
| Wieland I, Bohm M, Arden KC, Ammermuller T, Bogatz S, Viars CS, Rajewsky MF, Allelic deletion mapping on chromosome 5 in human carcinomas. Oncogene12:97-102 1996 |
| PubMed ID: 8552404 |
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| Hobson GM, Krahe R, Garcia E, Siciliano MJ, Funanage VL, Regional chromosomal assignments for four members of the MADS domain transcription enhancer factor 2 (MEF2) gene family to human chromosomes 15q26, 19p12, 5q14, and 1q12-q23. Genomics29:704-11 1995 |
| PubMed ID: 8575763 |
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| DiDonato CJ, Morgan K, Carpten JD, Fuerst P, Ingraham SE, Prescott G, McPherson JD, Wirth B, Zerres K, Hurko O, et al, Association between Ag1-CA alleles and severity of autosomal recessive proximal spinal muscular atrophy. Am J Hum Genet55:1218-29 1994 |
| PubMed ID: 7977383 |
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| Bernard LE, McPherson JD, Wasmuth JJ, Kreklywich CN, Brzustowicz LM, Wood S, Linkage mapping detects two secondary microdeletions in cell hybrid HHW1064, used to isolate DNA probes from within 5q11.2-->q13.3. Cytogenet Cell Genet64:46-48 1993 |
| PubMed ID: 8508678 |
| Split Ratio |
1:25 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
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