GM11440
Somatic cell hybrid
Description:
CHROMOSOME 5 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 7 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/Chinese hamster somatic cell hybrid retaining a human der(5)t(5;7) in 100% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 4p16.2p15.1, D4S20, and 4q21q23, ADH3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 5pterp15.3, D5S10, and gave a positive result with a probe for 5q33q35, PDGFRB. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 5q14-p12, C9, 5q11.2-q13.1, D5S351, 5q13.3-q14, HMGCR, 5q32, D5S372, and 5q34-q35, GABRA1, and gave a negative result with a primer for 5p15.3-p15.1, D5S208. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 7pterp15, ASSP11, and 7q31.1q31.2, D7S18. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 7p12, EGFR, 7q31.3, CFTR, and 7q36-qter, D7S550, and gave a positive result with a primer for 7pter-p22, D7S472. |
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| Remarks |
Line HHW1249; human/Chinese hamster somatic cell hybrid; grown in medium containing 0.2mM proline at 39C; retains human der(5)t(5;7)(p15.1;p22) in 100% of cells |
| Split Ratio |
1:25 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
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