GM11543
Somatic cell hybrid
Description:
CHROMOSOME 16 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
Chinese hamster/human hybrid retaining human der(16)t(16;17)(q23;q21), #6, 10, 18, 20, & Y
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 12q14q24.1, D12S7. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 14 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 14q32.1q32.3, D14S13. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 16pterp13, D16S36, and 16q13q22.1, MT2. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 16q22.1, LCAT, and 16q23.2, D16S266, and gave negative results with 2 primers for 16q24.3, D16S305 and PRSM1. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17p13.1, MYH2, and gave a positive result with a probe for 17q21.3q23, MPO. |
| |
| Remarks |
Line EDA5; produced by the fusion of human lymphoblasts, 46,XY,t(16;17)(q23; q21), with TK-deficient Chinese hamster a23 cells; grown in HAT culture medium; retains human der(16)t(16;17) in 100% of cells & other human chromosomes |
| Pelletier GJ, Brody SL, Liapis H, White RA, Hackett BP, A human forkhead/winged-helix transcription factor expressed in developing pulmonary and renal epithelium. Am J Physiol274:L351-9 1998 |
| PubMed ID: 9530170 |
| |
| Cerosaletti KM, Shapero MH, Fournier RE, Cloning mammary cell cDNAs from 17q12-q23 using interspecific somatic cell hybrids and subtractive hybridization. Genomics25:226-37 1995 |
| PubMed ID: 7774923 |
| |
| Kusuda J, Tanuma R, Gotoh E, Toyoda A, Hashimoto K, Assignment of a human autoimmune antigen, p80-coilin gene to chromosome 17q21-q23 and of its possible pseudogene to chromosome 14. Hum Genet95:233-4 1995 |
| PubMed ID: 7860074 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
|
|