GM11575
Somatic cell hybrid
Description:
CHROMOSOME 13 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Karyotypic analysis and Southern blot hybridization
|
|
ISCN
|
Human/Chinese hamster hybrid retaining human der(13)t(X;13) in 92% of cells, #22 in 52%, & X in 100%
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for Xp22.32 and Yp11.3, MIC2, and Xq28, DXS15. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp11.3, DXS1003, Xp22.32, STS, Xq12, DXS453, Xq13.3, PGK1, Xq21.3, DXS3, Xq26, HPRT, and Xq28, G6PD. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 1p36, PND. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 2p25, D2S1. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 6q24q27, ESR. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 10q24qter, PLAU. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 12p13.2, PRB1, and gave a negative result with a probe for 12q14q24.1, D12S7. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 13q34, D13S3. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 13q11-q12, D13S232, and gave negative results with primers for 13q14.3, RB1, 13q22-q31, D13S317, and 13q34, F7.1. |
| |
| Remarks |
Line X13-C9; produced by fusing lymphoblasts from a pt, GM09963, with retinoblastoma, 46,X,t(X;13)(q23;q13), with HPRT-def Chinese hamster line RJK88; selected in HZ medium; retains der(13) (92% cells) & other hum chromos includ X |
| Barr FG, Sellinger B, Emanuel BS, Localization of the rhabdomyosarcoma t(2;13) breakpoint on a physical map of chromosome 13. Genomics11:941-7 1991 |
| PubMed ID: 1783402 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Azaserine 5 x 10-4M |
| Supplement |
Hypoxanthine 1 x 10-4M |
|
|