GM11619
Somatic cell hybrid
Description:
CHROMOSOME 8 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Chinese hamster/human hybrid retaining a human del(8)(pter>8q12::) & other human chromosomes
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 8q22q23, D8S5, and 8q13q22.1, CA2, and gave a positive result with a probe for 8p21, NEFL. M. Wagner, Research Triangle Park, NC, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 8p22, D8S258, 8p21.3-cen, D8S259, and 8q11, MOS, and gave negative results with primers for 8q13-q22.1, D8S286, 8q22.2, D8S271, 8q22.3, D8S276, and 8q24.3-qter, D8S263. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 8q22.2, D8S270, 8q23-q24, D8S199, and 8q24, TG, and gave positive results with primers for 8p12, D8S87, 8p23-p22, DEF1, 8p21-p11.2, LHRH, 8q11, MOS, and 8q11.22-q12, D8S260. |
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| Remarks |
Line 1HL33; human/Chinese hamster ovary somatic cell hybrid retaining human 8pter>8q12 in 74% of cells; grown in medium without selection |
| Wagner MJ, Ge Y, Siciliano M, Wells DE, A hybrid cell mapping panel for regional localization of probes to human chromosome 8. Genomics10:114-25 1991 |
| PubMed ID: 2045096 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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