GM11937
Somatic cell hybrid
Description:
CHROMOSOME 11 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and Southern blot hybridization
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ISCN
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Human/Chinese hamster hybrid retaining a human der(11)t(4;11) & other human chromosomes including #4
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 2p25, D2S1, and 2q37, D2S3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 4p16.2p15.1, D4S20, and 4q21q23, ADH3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 7q31.1q31.2, D7S18, and 7pterp15, ASSP11. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 11p13, CAT, and 11p15.5, D11S12, and gave a negative result with a probe for 11q23.3, ETS1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 16q13q22.1, MT2, and gave a positive result with a probe for 16pterp13, D16S36. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. |
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| Remarks |
Line A3RS; produced by the fusion of lymphoblasts from a patientt with ALL, t(4;11)(q21; q23) and TK-deficient Chinese hamster ovary a3 cells; grown in medium without selection; retains der(11)t(4;11) in 72% of cells and other human chromosomes including #4 |
| Gabriel JM, Higgins MJ, Gebuhr TC, Shows TB, Saitoh S, Nicholls RD, A model system to study genomic imprinting of human genes. Proc Natl Acad Sci U S A95:14857-62 1998 |
| PubMed ID: 9843980 |
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| Junien C, van Heyningen V, Evans G, Little P, Mannens M, Report of the second chromosome 11 workshop. Genomics12:620-5 1992 |
| PubMed ID: 1348491 |
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| Cotter FE, Das S, Douek E, Carter NP, Young BD, The generation of DNA probes to chromosome 11q23 by Alu PCR on small numbers of flow-sorted 22q- derivative chromosomes. Genomics9:473-80 1991 |
| PubMed ID: 2032719 |
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| Tokino T, Takahashi E, Mori M, Tanigami A, Glaser T, Park JW, Jones C, Hori T, Nakamura Y, Isolation and mapping of 62 new RFLP markers on human chromosome 11. Am J Hum Genet48:258-68 1991 |
| PubMed ID: 1671318 |
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| Rowley JD, Diaz MO, Espinosa R 3d, Patel YD, van Melle E, Ziemin S, Taillon-Miller P, Lichter P, Evans GA, Kersey JH, et al, Mapping chromosome band 11q23 in human acute leukemia with biotinylated probes: identification of 11q23 translocation breakpoints with a yeast artificial chromosome. Proc Natl Acad Sci U S A87:9358-62 1990 |
| PubMed ID: 2251277 |
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| Savage PD, Jones C, Silver J, Geurts van Kessel AH, Gonzalez-Sarmiento R, Palm L, Hanson CA, Kersey JH, Mapping studies and expression of genes located on human chromosome 11, band q23. Cytogenet Cell Genet49:289-92 1988 |
| PubMed ID: 3248386 |
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| Sacchi N, Watson DK, Guerts van Kessel AH, Hagemeijer A, Kersey J, Drabkin HD, Patterson D, Papas TS, Hu-ets-1 and Hu-ets-2 genes are transposed in acute leukemias with (4;11) and (8;21) translocations. Science231:379-82 1986 |
| PubMed ID: 3941901 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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