GM12506
Somatic cell hybrid
Description:
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/mouse hybrid retaining human del(17) in 96% of cells & #6 (92%), 8 (96%), 12 (84%), 18 (20%), & X (8%)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this cell culture gave a negative result with a primer for Yq11, DYS227. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 17p13.1, TP53, 17q, GAS, and 17q21.3-q23, MPO. |
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| Remarks |
Line Ag-2; produced by the fusion of human cells from a pt with Miller-Dieker syndrome with TK-deficient Cl1D mouse cells; grown in HAT medium; retains human del(17)(p13.3) in 96% of cells & other human chromosomes |
| Roizès G, Human centromeric alphoid domains are periodically homogenized so that they vary substantially between homologues Mechanism and implications for centromere functioning Nucleic acids research34:1912-24 2006 |
| PubMed ID: 16598075 |
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| Small KM, Brown KM, Seman CA, Theiss CT, Liggett SB, Complex haplotypes derived from noncoding polymorphisms of the intronless alpha2A-adrenergic gene diversify receptor expression Proceedings of the National Academy of Sciences of the United States of America103:5472-7 2006 |
| PubMed ID: 16567612 |
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| Ledbetter DH, Ledbetter SA, vanTuinen P, Summers KM, Robinson TJ, Nakamura Y, Wolff R, White R, Barker DF, Wallace MR, et al, Molecular dissection of a contiguous gene syndrome: frequent submicroscopic deletions, evolutionarily conserved sequences, and a hypomethylated "island" in the Miller-Dieker chromosome region. Proc Natl Acad Sci U S A86:5136-40 1989 |
| PubMed ID: 2740347 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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