GM12508
Somatic cell hybrid
Description:
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/mouse hybrid retaining human del(17) & del(11) (84% of cells) & #15 (80% of cells)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
FISH performed at the CCR with pooled probes for the Miller-Dieker critical region and chromosome 17 alpha satellite DNA consistently identified a human chromosome 17 lacking the Miller-Dieker critical region in this human/mouse somatic cell hybrid. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 17p13.1, TP53, 17q21.3-q23, MPO1, 17q, GAS, and 17q25-qter, D17S784. |
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| Remarks |
Line Br-8; produced by the fusion of human cells from a pt with Miller-Dieker syndrome with TK-def Cl1D mouse cells; grown in HAT medium; retains human del (17)(p13.3) in 100% of cells & other human chromosomes {del(11) & #15} |
| Ledbetter DH, Ledbetter SA, vanTuinen P, Summers KM, Robinson TJ, Nakamura Y, Wolff R, White R, Barker DF, Wallace MR, et al, Molecular dissection of a contiguous gene syndrome: frequent submicroscopic deletions, evolutionarily conserved sequences, and a hypomethylated "island" in the Miller-Dieker chromosome region. Proc Natl Acad Sci U S A86:5136-40 1989 |
| PubMed ID: 2740347 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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