GM12510
Somatic cell hybrid
Description:
CHROMOSOME X REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Chinese hamster/human hybrid retaining a human der(17)t(X;17), #8, & 18
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for Xp22.32 and Yp11.3, MIC2, and gave a positive result with a probe for Xq28, DXS15. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for Xp11.21, ALAS2, Xp11.3, DXS1003, Xp22.32, STS, Xq12, DXS453, Xq13.3, PGK1, and Xq21.3, DXS3, and gave positive results with primers for Xq26, HPRT, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 15q25qter, IGF1R. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17p13.1, MYH2, and gave a positive result with a probe for 17q21.3q23, MPO. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 17q21.3-q23, MPO. |
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| Remarks |
Line CAIA2; produced by the fusion of human cells from a Sotos syndrome patient, 46,X,t(X;17)(q22;p11.2), with HPRT-deficient Chinese hamster RJK88 cells; grown in HZ medium; retains human der(17) in 84% of cells and other human chromosomes |
| Hotzel I, Cheevers WP, A maedi-visna virus strain K1514 receptor gene is located in sheep chromosome 3p and the syntenic region of human chromosome 2. J Gen Virol83(Pt 7):1759-64 2002 |
| PubMed ID: 12075096 |
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| Harun RB, Smith KK, Leek JP, Markham AF, Norris A, Morrison JF, Characterization of human SHC p66 cDNA and its processed pseudogene mapping to Xq12-q13.1. Genomics42:349-52 1997 |
| PubMed ID: 9192859 |
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| Plummer SJ, Simmons JA, Adams L, Casey G, Mapping of 228 ESTs and 26 genes into an integrated physical and genetic map of human chromosome 17. Genomics45:140-6 1997 |
| PubMed ID: 9339370 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Azaserine 5 x 10-3M |
| Supplement |
Hypoxanthine 4 x 10-3M |
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