GM12517
Somatic cell hybrid
Description:
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Mouse/human hybrid retaining a human i(17q), 18, & 21
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17p13.1, MYH2, and gave a positive result with a probe for 17q21.3q23, MPO. |
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| Remarks |
Line LS-1; produced by the fusion of human cells from a pt with t(15;17)(q13; p13.3) with thymidine kinase-deficient mouse Cl1D cells; grown in HAT medium; retains human i(17q) in 96% of cells, #18 (4% of cells) & #21 (84% of cells) |
| Bloch KD, Wolfram JR, Brown DM, Roberts JD Jr, Zapol DG, Lepore JJ, Filippov G, Thomas JE, Jacob HJ, Bloch DB, Three members of the nitric oxide synthase II gene family (NOS2A, NOS2B, and NOS2C) colocalize to human chromosome 17. Genomics27:526-30 1995 |
| PubMed ID: 7558036 |
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| van Tuinen P, Rich DC, Summers KM, Ledbetter DH, Regional mapping panel for human chromosome 17: application to neurofibromatosis type 1. Genomics1:374-81 1987 |
| PubMed ID: 3130306 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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