Repository NIGMS Human Genetic Cell Repository
Subcollection CEPH
Repository Linkage Families
Quantity 0.050mg
Quantitation Method Please see our FAQ
Biopsy Source Peripheral vein
Cell Type B-Lymphocyte
Tissue Type Blood
Transformant Epstein-Barr Virus
Ethnicity FRENCH
Family Member 4
Relation to Proband daughter
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks Daughter
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis
Remark Daughter
Beckman KB, Abel KJ, Braun A, Halperin E, Using DNA pools for genotyping trios Nucleic Acids Research34(19):e129 2006
PubMed ID: 17020923
Wu WM, Tsai HJ, Pang JH, Wang TH, Wang HS, Hong HS, Lee YS, Linear allele-specific long-range amplification: a novel method of long-range molecular haplotyping Human mutation26:393-4 2005
PubMed ID: 16134125
Sebat J, Lakshmi B, Troge J, Alexander J, Young J, Lundin P, Maner S, Massa H, Walker M, Chi M, Navin N, Lucito R, Healy J, Hicks J, Ye K, Reiner A, Gilliam TC, Trask B, Patterson N, Zetterberg A, Wigler M, Large-scale copy number polymorphism in the human genome. Science305(5683):525-8 2004
PubMed ID: 15273396
Antson DO, Mendel-Hartvig M, Landegren U, Nilsson M, PCR-generated padlock probes distinguish homologous chromosomes through quantitative fluorescence analysis European journal of human genetics : EJHG11:357-63 2003
PubMed ID: 12734539
View pedigree 
Split Ratio 1:5
Temperature 37 C
Percent CO2 5%
Medium Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not Inactivated
Substrate None specified
Subcultivation Method dilution - add fresh medium