GM13158
Somatic cell hybrid
Description:
CHROMOSOME 16 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 10 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/mouse somatic cell hybrid retaining a human der(10)t(10;16) & #8
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 1q32, REN. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 8p23-p22, DEF1, 8p21-p11.2, LHRH, and 8q24, TG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 10p11.2, ITGB1, and 10q24, PLAU. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 16p13.3, HBA, and gave positive results with primers for 16q22.1, LCAT, 16q23.2, D16S266, and 16q24.3, PRSM1. |
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| Remarks |
Line CY6; produced by the fusion of human cells, 46,XX,t(10;16)(q24.3;q22.1), with mouse A9 cells; grown in medium with alanosine & adenine; retains human der(10)t(10;16) in 96% of cells & #8 in 64% of cells |
| Oh J, Bailin T, Fukai K, Feng GH, Ho L, Mao JI, Frenk E, Tamura N, SpritzRA, Positional cloning of a gene for Hermansky-Pudlak syndrome, a disorder of
cytoplasmic organelles. Nat Genet14(3):300-6 1996 |
| PubMed ID: 8896559 |
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| Callen DF, Doggett NA, Stallings RL, Chen LZ, Whitmore SA, Lane SA, Nancarrow JK, Apostolou S, Thompson AD, Lapsys NM, et al, High-resolution cytogenetic-based physical map of human chromosome 16. Genomics13:1178-85 1992 |
| PubMed ID: 1505951 |
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| Callen DF, Hyland VJ, Baker EG, Fratini A, Simmers RN, Mulley JC, Sutherland GR, Fine mapping of gene probes and anonymous DNA fragments to the long arm of chromosome 16. Genomics2:144-53 1988 |
| PubMed ID: 2900808 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Adenine 5 x 10-2mM |
| Supplement |
Azaserine 5 x 10-2mM |
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