GM13332
Somatic cell hybrid
Description:
CHROMOSOME 5 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 4 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Chinese hamster/human hybrid retaining human chromosomes 3, 18, 21, 22, & der(4)t(4;5)(q12;q22)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 3p25, RAF1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 4 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 4p16.2p15.1, D4S20, and gave a negative result with a probe for 4q21q23, ADH3. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 4p15.3, QDPR, 4p16.3, PDEB.2, and 4p16.3, D4S126, and gave negative results with primers for 4q11-q13, AFP, 4q25, EGF, 4q28, FGA, 4q31-q33, D4S1626, and 4q35, F11. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 5pterp15.3, D5S10, and gave a positive result with a probe for 5q33q35, PDGFRB. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 5p14-p12, C9, 5p15.3-p15.1, D5S208, 5q11.2q13.1, D5S351, and 5q13.3-q14, HMGCR, and gave positive results with primers for 5q32, D5S372, and 5q34-q35, GABRA1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 18q23, MBP. |
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| Remarks |
Line HHW1499; human/Chinese hamster hybrid; retains human der(4)t(4;5)(4pter> 4q12::5q22>5qter); grown in culture medium containing 0.2mM proline at 39C; 100% of cells have the der(4)t(4;5), #3 (84%), #18 (84%), #21 (100%) & #22 (24%) |
| Inazawa J, Ariyama T, Abe T, Druck T, Ohta M, Huebner K, Yanagisawa J, Reed JC, Sato T, PTPN13, a fas-associated protein tyrosine phosphatase, is located on the long arm of chromosome 4 at band q21.3. Genomics31:240-2 1996 |
| PubMed ID: 8824809 |
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| Slaugenhaupt SA, Roca AL, Liebert CB, Altherr MR, Gusella JF, Reppert SM, Mapping of the gene for the Mel1a-melatonin receptor to human chromosome 4 (MTNR1A) and mouse chromosome 8 (Mtnr1a). Genomics27:355-7 1995 |
| PubMed ID: 7558006 |
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| McPherson JD, Morton RA, Ewing CM, Wasmuth JJ, Overhauser J, Nagafuchi A, Tsukita S, Isaacs WB, Assignment of the human alpha-catenin gene (CTNNA1) to chromosome 5q21- q22. Genomics19:188-90 1994 |
| PubMed ID: 8188230 |
| Split Ratio |
1:5 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
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