GM13445
Somatic cell hybrid
Description:
CHROMOSOME 6 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 7 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Karyotypic analysis and In situ hybridization
|
|
ISCN
|
Mouse/human hybrid retaining a human der(7)t(6;7) in 80% of cells & human #3, 10, 13, 14, 17, 19, 21, & 22
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 6 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 6p21.3, TNFA, and 6p25, D6S344, and gave positive results with primers for 6q21-q23.3, D6S292, 6q24-q27, MAS1, and 6q27-qter, D6S297. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 7p12, EGFR, and 7pter-p22, D7S472, and gave negative results with primers for 7q31.3, CFTR, and 7q36-qter, D7S550. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 10q24, PLAU. |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 22q11.2, IGLC2. |
| |
| Remarks |
Line 5184-4; produced by the fusion of human lymphoblasts, GM05184, 46,XY, t(6;7)(q21;q21.2) with TK-deficient mouse Cl1D cells; selected in HAT medium; retains der(7)t(6;7) in 80% of cells & other human chromosomes |
| Pappas GJ, Polymeropoulos MH, Boyle JM, Trent JM, Regional assignment by hybrid mapping of 36 expressed sequence tags (ESTs) on human chromosome 6. Genomics25:124-9 1995 |
| PubMed ID: 7774909 |
| |
| Meese EU, Meltzer PS, Ferguson PW, Trent JM, Alu-PCR: characterization of a chromosome 6-specific hybrid mapping panel and cloning of chromosome-specific markers. Genomics12:549-54 1992 |
| PubMed ID: 1559706 |
| |
| Meese EU, Witkowski CM, Zoghbi HY, Stanbridge EJ, Meltzer PS, Trent JM, Development and utilization of a somatic cell hybrid mapping panel to assign NotI linking probes to the long arm of human chromosome 6. Genomics12:542-8 1992 |
| PubMed ID: 1559705 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
|
|