GM13492
Somatic cell hybrid
Description:
CHROMOSOME X REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 2 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Chinese hamster/human hybrid retaining human der(2)t(X;2) in 88% of cells & #6, 13, 16, 21, & 22
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for Xp11.21, ALAS2, Xp11.3, DXS1003, Xp22.32, STS, and Xq12, DXS453, and gave positive results with primers for Xq13.3, PGK1, Xq21.3, DXS, Xq26, HPRT, Xq28, G6PD, and Xq23, DXS1059. |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave a negtive result with a primer for Yq11, DYS227. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 2q37, ALPP, and gave positive results with primers for 2p13, TGFA, 2p23, POMC, and 2q13, IL1A. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 22q11.2, IGLC2. |
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| Remarks |
Line HCG10; produced by the fusion of hum lymphos from a Menkes syn pt, 46,X, t(X;2)(q13.3;q32.2) with HPRT-def RJK88 Chinese hamster cells; med contains hypoxanthine & azaserine; 88% of cells retain the der(2)t(X;2) & other hum chromo |
| Kohler A, Logan C, Joyner AL, Muenke M, Regional assignment of the human homeobox-containing gene EN1 to chromosome 2q13-q21. Genomics15:233-5 1993 |
| PubMed ID: 8094370 |
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| Mercer JF, Livingston J, Hall B, Paynter JA, Begy C, Chandrasekharappa S, Lockhart P, Grimes A, Bhave M, Siemieniak D, et al, Isolation of a partial candidate gene for Menkes disease by positional cloning [see comments] Nat Genet3:20-5 1993 |
| PubMed ID: 8490647 |
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| Verga V, Hall BK, Wang SR, Johnson S, Higgins JV, Glover TW, Localization of the translocation breakpoint in a female with Menkes syndrome to Xq13.2-q13.3 proximal to PGK-1. Am J Hum Genet48:1133-8 1991 |
| PubMed ID: 2035533 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Azaserine 10-5M |
| Supplement |
Hypoxanthine 10-4M |
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