GM13501
Somatic cell hybrid
Description:
CHROMOSOME 22 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 12 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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Human/Chinese hamster somatic cell hybrid retaining human 12cen>12q13.31:: 22q13.31>22qter (60%) & hu marker chroms
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for Yq11, DYS227. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 10 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 10p11.2, ITGB1, and gave a negative result with a primer for 10q24, PLAU. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 12p12-p11, D12S1042, 12q13, D12S371, 12q13.3q14, D12S191, 12q22-q23, IGF1, 12q22-q24.2, PAH, and 12q24-qter, D12S392. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 22q11.2, IGLC2, 22q12.1-q13.1, D22S300, and 22q13.31-qter, DIA1, and gave a positive result with a primer for 22q13.31, KI-108. |
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| Remarks |
Line Cl-2/DIBA; human/Chinese hamster hybrid retaining human 12cen>12q13.31::22q13.31>22qter in 60% of cells by dual probe FISH analysis and many human markers; grown in medium with thymidine |
| Bell CJ, Budarf ML, Nieuwenhuijsen BW, Barnoski BL, Buetow KH, Campbell K, Colbert AM, Collins J, Daly M, Desjardins PR, et al, Integration of physical, breakpoint and genetic maps of chromosome 22. Localization of 587 yeast artificial chromosomes with 238 mapped markers. Hum Mol Genet4:59-69 1995 |
| PubMed ID: 7711735 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Thymidine 1 x 10-5M |
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