GM14064
Somatic cell hybrid
Description:
CHROMOSOME 8 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 21 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/Chinese hamster hybrid retaining a human der(8)t(8;21)(q22.1;q22.3)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 8p12, D8S87, 8p23-p22, DEF1, 8p21-p11.2, LHRH, 8q11, MOS, 8q11.22-q12, D8S260, and 8q22.2, D8S270, and gave negative results with primers for 8q23-q24, D8S199, and 8q24,TG. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 21q21.2, APP, and 21q22.3, ITGB2, and gave negative results with primers for 21p11.2, D21S1278, 21q11, D21S120, 21q11.1, D21S236, and 21q21.2, AMYLOID. |
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| Remarks |
Line 8q-a; produced by the fusion of human cells, t(8;21)(q22.1;q22.3), from an AML-M2 patient with Chinese hamster ovary Ade-C cells; grown in HF12 medium with hypoxanthine; retains human der(8) t(8;21) in ??% of cells |
| Chastain-Moore AM, Roberts T, Trott DA, Newbold RF, Ornelles DA, An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression. J Virol77(14):8087-98 2003 |
| PubMed ID: 12829847 |
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| Wagner MJ, Ge Y, Siciliano M, Wells DE, A hybrid cell mapping panel for regional localization of probes to human chromosome 8. Genomics10:114-25 1991 |
| PubMed ID: 2045096 |
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| Gardiner K, Horisberger M, Kraus J, Tantravahi U, Korenberg J, Rao V, Reddy S, Patterson D, Analysis of human chromosome 21: correlation of physical and cytogenetic maps; gene and CpG island distributions. EMBO J9:25-34 1990 |
| PubMed ID: 2295313 |
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| Gardiner K, Watkins P, Munke M, Drabkin H, Jones C, Patterson D, Partial physical map of human chromosome 21. Somat Cell Mol Genet14:623-37 1988 |
| PubMed ID: 3194801 |
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| Sacchi N, Cheng SV, Tanzi RE, Gusella JF, Drabkin HA, Patterson D, Haines JH, Papas TS, The ETS genes on chromosome 21 are distal to the breakpoint of the acute myelogenous leukemia translocation (8;21). Genomics3:110-6 1988 |
| PubMed ID: 3267212 |
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| Sacchi N, Watson DK, Guerts van Kessel AH, Hagemeijer A, Kersey J, Drabkin HD, Patterson D, Papas TS, Hu-ets-1 and Hu-ets-2 genes are transposed in acute leukemias with (4;11) and (8;21) translocations. Science231:379-82 1986 |
| PubMed ID: 3941901 |
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| Diaz MO, Le Beau MM, Rowley JD, Drabkin HA, Patterson D, The role of the c-mos gene in the 8;21 translocation in human acute myeloblastic leukemia. Science229:767-9 1985 |
| PubMed ID: 3860954 |
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| Drabkin HA, Diaz M, Bradley CM, Le Beau MM, Rowley JD, Patterson D, Isolation and analysis of the 21q+ chromosome in the acute myelogenous leukemia 8;21 translocation: evidence that c-mos is not translocated. Proc Natl Acad Sci U S A82:464-8 1985 |
| PubMed ID: 2982159 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
5% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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