GM14111
Somatic cell hybrid
Description:
CHROMOSOME 5 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/Chinese hamster hybrid retaining a human der(5)t(3;5)(q29;p15.1), #9, & 21
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 3p14.1-p12, D3S1566, 3p22-p21.33, GLB1, 3p25p24.2, D3S1304, 3q22-q24, D3S1764, 3q26.2-q27, GLUT2, and 3q28-q29, D3S1311. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 5p14-p12, C9, 5p15.3-p15.1, D5S208, 5q11.2q13.1, D5S351, 5q13.3-q14, HMGCR, 5q32, D5S372, and 5q34-q35, GABRA1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 17p13.1, TP53. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 21p11.2, D21S1278, 21q11, D21S120, 21q11.1, D21S236, and 21q21.1, AMYLOID. |
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| Remarks |
Line JH148; human lymphoblasts, GM14119, t(3;5)(p15.1;q29) fused with Chinese hamster UCW56 cells; grown in medium containing 0.2mM proline at 39C; retains human der(5)t(3;5)(q29;p15.1) in 100% of cells and chromosomes 9 and 21 |
| Overhauser J, Huang X, Gersh M, Wilson W, McMahon J, Bengtsson U, Rojas K, Meyer M, Wasmuth JJ, Molecular and phenotypic mapping of the short arm of chromosome 5: sublocalization of the critical region for the cri-du-chat syndrome. Hum Mol Genet3:247-52 1994 |
| PubMed ID: 8004090 |
| Split Ratio |
1:5 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
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