GM14216
Somatic cell hybrid
Description:
CHROMOSOME 15 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Chinese hamster somatic cell hybrid
rev ish enh (5, 7, 9q22>9q31, 15q22>15q25)
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for Xp22.32, STS, and Xq21.3, DXS3. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 2 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 2p13, TGFA. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 3q28-q29, D3S1311. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 5p14-p12, C9, 5q13.3-q14, HMGCR, 5q34-q35, GABRA1, and 5q21-q22, MCC. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 7 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 7pter-p22, D7S472. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 15 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 15q23, D15S1485A, and gave a negative result with a primer for 15q21-q22.2, B2M. |
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| Remarks |
Line HHW1126; produced by fusion of human cells, GM14217, with Chinese hamster cells; grown in medium with 0.2mM proline at 39C; retains a human der(5) ins (5;15)(q23.2;q22q25) in ??% of cells & other human chromosomes |
| Loftus SK, Shiang R, Warrington JA, Bengtsson U, McPherson JD, Wasmuth JJ, Genes encoding adrenergic receptors are not clustered on the long arm of human chromosome 5. Cytogenet Cell Genet67:69-74 1994 |
| PubMed ID: 8039425 |
| Split Ratio |
1:5 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 34.5mg/L |
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