GM14227
Somatic cell hybrid
Description:
CHROMOSOME 18 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Chinese hamster hybrid retaining a human der(18)t(18;21)(p11.21;q11.2) in 92% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 18 |
PCR analysis of DNA from this somatic cell hybrid gave a negative result with a primer for 18p11.31-p11.22, TYMS, and gave positive results with primers for 18p11.1-q11.2, D18S44, 18q12.1, TTR, and 18q23qter, D18S844. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 21q11, D21S120, 21q11.1, D21S236, 21q21.2, APP, 21q21.2, AMYLOID, and 21q22.3, ITGB2, and gave negative results with primers for 21p11.2, pAW14a, and 21p11.2, D21S1278. |
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| Remarks |
Line HHW778; produced by the fusion of human cells with Chinese hamster UCW206 cells, GM14228; grown in medium with 0.2mM proline at 39C; retains a human der(18)t(18;21)(p11;q11.2) in 92% of cells |
| Chastain-Moore AM, Roberts T, Trott DA, Newbold RF, Ornelles DA, An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression. J Virol77(14):8087-98 2003 |
| PubMed ID: 12829847 |
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| Kline AD, Rojas K, Mewar R, Moshinsky D, Overhauser J, Somatic cell hybrid deletion map of human chromosome 18. Genomics13:1-6 1992 |
| PubMed ID: 1577474 |
| Split Ratio |
1:5 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 34.5mg/L |
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