GM14462
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 5 SOMATIC CELL HYBRIDS
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Hybrids |
Cell Type
|
Somatic cell hybrid
|
Transformant
|
Untransformed
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
Human/Chinese hamster hybrid retaining a human del(5)(p14.3p14.1) in 100% of cells, 6, 8, 13, 18, 19, and 20
|
Remarks
|
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
|
Remarks |
Line HHW980; produced by the fusion of human lymphoblasts with Chinese hamster cells; grown in medium with 0.2mM proline at 39C; retains a human del(5) (p14.1p14.3) in ??% of cells & other human chromosomes |
Split Ratio |
1:5 |
Temperature |
39 C |
Percent CO2 |
8% |
Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Proline 34.5mg/L |
|
|