GM14963
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 16 SOMATIC CELL HYBRIDS
Repository
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NIGMS Human Genetic Cell Repository
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Subcollection |
Hybrids |
Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis before cell line submission to CCR
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ISCN
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Human/mouse somatic cell hybrid retaining a human der(15)t(15;16)(q26.1;q22.1) in 88% of cells, 4, 13, and 15
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Remarks
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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Remarks |
Line CY124; produced by the fusion of human fibroblast line , 46,XX,t(15;16)(q26.1;q22.1), with APRT deficient mouse A9 cells; retains a human der(15)t(15;16)(q26.1;q22.1) in 88% of cells and a human 4, 13, and 15 |
Callen DF, Doggett NA, Stallings RL, Chen LZ, Whitmore SA, Lane SA, Nancarrow JK, Apostolou S, Thompson AD, Lapsys NM, et al, High-resolution cytogenetic-based physical map of human chromosome 16. Genomics13:1178-85 1992 |
PubMed ID: 1505951 |
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Richards RI, Holman K, Lane S, Sutherland GR, Callen DF, Human chromosome 16 physical map: mapping of somatic cell hybrids using multiplex PCR deletion analysis of sequence tagged sites. Genomics10(4):1047-52 1991 |
PubMed ID: 1916811 |
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Callen DF, Baker E, Eyre HJ, Lane SA, An expanded mouse-human hybrid cell panel for mapping human chromosome 16. Ann Genet33:190-5 1990 |
PubMed ID: 2095701 |
Split Ratio |
1:8 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Adenine 5 x 10-2mM |
Supplement |
Azaserine 5 x 10-2mM |
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