Repository NIGMS Human Genetic Cell Repository
Subcollection Polymorphism Discovery Resource
Pharmacogenetics
Quantity 0.025mg
Quantitation Method Please see our FAQ
Biopsy Source Peripheral vein
Cell Type B-Lymphocyte
Tissue Type Blood
Transformant Epstein-Barr Virus
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis
 
No data is available
Wyrobek AJ, Manohar CF, Krishnan VV, Nelson DO, Furtado MR, Bhattacharya MS, Marchetti F, Coleman MA, Low dose radiation response curves, networks and pathways in human lymphoblastoid cells exposed from 1 to 10cGy of acute gamma radiation Mutation research722:119-30 2011
PubMed ID: 21497671
 
Asur R, Balasubramaniam M, Marples B, Thomas RA, Tucker JD, Involvement of MAPK proteins in bystander effects induced by chemicals and ionizing radiation Mutation research686:15-29 2009
PubMed ID: 20034502
 
Koukouritaki SB, Poch MT, Cabacungan ET, McCarver DG, Hines RN, Discovery of novel flavin-containing monooxygenase 3 (FMO3) single nucleotide polymorphisms and functional analysis of upstream haplotype variants Molecular pharmacology68:383-92 2005
PubMed ID: 15858076
 
Coleman MA, Yin E, Peterson LE, Nelson D, Sorensen K, Tucker JD, Wyrobek AJ, Low-dose irradiation alters the transcript profiles of human lymphoblastoid cells including genes associated with cytogenetic radioadaptive response Radiation research164:369-82 2005
PubMed ID: 16187739
 
Deshpande A, Nolan JP, White PS, Valdez YE, Hunt WC, Peyton CL, Wheeler CM, TNF-alpha promoter polymorphisms and susceptibility to human papillomavirus 16-associated cervical cancer The Journal of infectious diseases191:969-76 2004
PubMed ID: 15717274
 
Hines RN, Luo Z, Hopp KA, Cabacungan ET, Koukouritaki SB, McCarver DG, Genetic variability at the human FMO1 locus: significance of a basal promoter yin yang 1 element polymorphism (FMO1*6) The Journal of pharmacology and experimental therapeutics306:1210-8 2003
PubMed ID: 12829732
No data is available
Split Ratio 1:4
Temperature 37 C
Percent CO2 5%
Medium Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not Inactivated
Substrate None specified
Subcultivation Method dilution - add fresh medium