GM20435
LCL from B-Lymphocyte
Description:
DICENTRIC CHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities
dbGaP |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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46,X,dic(Y)(q11.221)[44]/45,X[6].ish dic(Y)(q11.221)(DXYS129/DXYS153++,SRY++,DYZ3++,DYZ1-,Z43206-).arr Yp11.31p11.221(2710425-18073907)x2,Yq11.222q12(19074170-27209311)x0,6q26(162289846-162395046)x1
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
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| Remarks |
Phenotypically normal; 45,X/46,X,del(Y)(q11) mosaicism diagnosed via amniocentesis during mother's pregnancy; normal physical exam with no dysmorphic features and no stigmata of Turner syndrome at age 2 months; normal physical exam with no dysmorphic features at age 13 months; normal genitalia with both testes descended and of normal size; the following Y chromosome loci were analyzed by PCR: SRY+, RPS4Y+, ZFY+, PRKY+, AMELY+, TSPY+, KALP+, RBMY1-, DYZ1-, and DYZ2-; father is GM20437 |
| Tang Z, Berlin DS, Toji L, Toruner GA, Beiswanger C, Kulkarni S, Martin CL, Emanuel BS, Christman M, Gerry NP, A dynamic database of microarray-characterized cell lines with various cytogenetic and genomic backgrounds G3 (Bethesda, Md)3:1143-9 2013 |
| PubMed ID: 23665875 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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