GM21880
Fibroblast from Skin, Unspecified
Description:
TRANSLOCATED CHROMOSOME
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Chromosome Abnormalities |
Biopsy Source
|
Unspecified
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Unspecified
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
ISCN
|
46,XY,t(4;6)(p14;q21).ish t(4;6)(wcp4+,wcp6+;wcp4+,wcp6+)
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
PDL at Freeze |
4.9 |
Passage Frozen |
5 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
|
Cytogenetics |
Chromosome 4: TRANSLOCATION Breakpoint 4p14 |
|
Chromosome 6: TRANSLOCATION Breakpoint 6q21 |
View |
FISH Spectrum Green = wcp4 ; Spectrum Orange = wcp6 |
|
FISH Spectrum Green = wcp4 ; Spectrum Orange = wcp6 |
|
karyotype Spectrum Green = wcp4 ; Spectrum Orange = wcp6 |
|
karyotype Spectrum Green = wcp4 ; Spectrum Orange = wcp6 |
Passage Frozen |
5 |
Split Ratio |
1:3 |
Temperature |
37 C |
Percent CO2 |
10% |
Percent O2 |
AMBIENT |
Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Supplement |
- |
|
|