GM22222
Fibroblast from Skin, Foreskin
Description:
MULTIPLE CELL TYPES FROM SAME SUBJECT - FORESKIN
APPARENTLY HEALTHY INDIVIDUAL
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Apparently Healthy Collection |
Biopsy Source
|
Foreskin
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Foreskin
|
Race
|
Asian
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
46,XY
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
PDL at Senescence |
26 |
Passage Frozen |
1 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
|
Remarks |
Foreskin fibroblast cell culture; another culture from this same donor is GM22221 (keratinocyte) |
Hossain MA, Hasegawa-Ogawa M, Manome Y, Igarashi M, Wu C, Suzuki K, Igarashi J, Iwamoto T, Okano HJ, Eto Y, Generation and characterization of motor neuron progenitors and motor neurons using metachromatic leukodystrophy-induced pluripotent stem cells Molecular genetics and metabolism reports31:100852 2022 |
PubMed ID: 35782608 |
PDL at Senescence |
26 |
Passage Frozen |
1 |
Split Ratio |
1:20 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
AMBIENT |
Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum |
Substrate |
None specified |
Supplement |
- |
|
|