GM23720
iPSC from B-Lymphocyte
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently healthy iPSCs
Apparently Healthy Collection |
| Protocols |
Protocol PDF |
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Biopsy Source
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Peripheral vein
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Cell Type
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Stem cell
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Tissue Type
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Induced pluripotent stem cell
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Transformant
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Reprogrammed (Episomal)
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis and Case history
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ISCN
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46,XX[20].arr[hg19]Xp21.1(31936202_32163760)x1, 16q11.2q24.3(46534976_90163275) hmz
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
51 |
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| Induced Pluripotent Stem Cell |
The cell line submitted to the Repository frozen was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and the PluriTest. Steady-state mRNA expression patterns of undifferentiated iPSCs and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
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| Remark |
Induced pluripotent stem cell line derived from GM02254 Lymphoid. Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is WARF. |
| Lee SI, Jeong W, Lim H, Cho S, Lee H, Jang Y, Cho J, Bae S, Lin YT, Tsai LH, Moon DW, Seo J, APOE4-carrying human astrocytes oversupply cholesterol to promote neuronal lipid raft expansion and Aß generation Stem cell reports16:2128-2137 2021 |
| PubMed ID: 34450034 |
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| Cho S, Lee H, Jung M, Hong K, Woo SH, Lee YS, Kim BJ, Jeon MY, Seo J, Mun JY, Neuromyelitis optica (NMO)-IgG-driven organelle reorganization in human iPSC-derived astrocytes FASEB journal : official publication of the Federation of American Societies for Experimental Biology35:e21894 2021 |
| PubMed ID: 34460995 |
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| Bogacheva MS, Bystriakova MA, Lou YR, Thyroid Hormone Effect on the Differentiation of Human Induced Pluripotent Stem Cells into Hepatocyte-Like Cells Pharmaceuticals (Basel, Switzerland)14:e21894 2021 |
| PubMed ID: 34200130 |
| Passage Frozen |
51 |
| Split Ratio |
1:15 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
mTeSR1 |
| Serum |
none |
| Substrate |
Matrigel |
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