GM25256
iPSC from Fibroblast
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently healthy iPSCs
Apparently Healthy Collection |
| Protocols |
Protocol PDF |
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Biopsy Source
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Skin
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Cell Type
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Stem cell
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Cell Subtype
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Induced pluripotent stem cell
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Transformant
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Reprogrammed (Episomal)
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Sample Source
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iPSC from Fibroblast
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Race
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Asian
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Ethnicity
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Not Hispanic/Latino
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Ethnicity
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Japanese
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Country of Origin
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USA
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Family Member
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1
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Family History
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N
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis before cell line submission to CCR
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ISCN
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46,XY[20]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
40 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
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| Induced Pluripotent Stem Cell |
The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
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| Remarks |
Induced pluripotent stem cell line derived from adult skin (leg) fibroblasts; subject clinically normal; wild-type; FISH test: 46, XY. This iPSC line (WTC-11) was submitted by Dr. Bruce R. Conklin (Gladstone Institute of Cardiovascular Disease, UCSF) and may serve as a control for diseased iPSCs GM25267 (LQT2) iPSC and GM25305 (LQT3); also available is a collection of fluorescently tagged hiPSC lines from the same subject, using CRISPR/Cas9 technology (for more information, see the Allen Cell Collection at Coriell: https://catalog.coriell.org/1/AllenCellCollection). Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is iPS Academia, Japan Inc.. |
| Barreto-Gamarra C, Domenech M, Integrin stimulation by collagen I at the progenitor stage accelerates maturation of human iPSC-derived cardiomyocytes Journal of molecular and cellular cardiology201:70-86 2025 |
| PubMed ID: 40023481 |
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| Hilal N, An Z, Prondzynski M, Matsui E, Sahu D, Mao S, Jung YL, Yang Y, Epstein S, Chen MH, Pu W, Monte FD, Huang AY, Choudhury S, Somatic Genomic and Transcriptomic Changes in Single Ischemic Human Heart Cardiomyocytes Research square201:70-86 2025 |
| PubMed ID: 39975917 |
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| Holmes BB, Weigel TK, Chung JM, Kaufman SK, Apresa BI, Byrnes JR, Kumru KS, Vaquer-Alicea J, Gupta A, Rose IVL, Zhang Y, Nana AL, Alter D, Grinberg LT, Spina S, Leung KK, Condello C, Kampmann M, Seeley WW, Coutinho-Budd JC, Wells JA, ß-Amyloid Induces Microglial Expression of GPC4 and APOE Leading to Increased Neuronal Tau Pathology and Toxicity bioRxiv : the preprint server for biology201:70-86 2025 |
| PubMed ID: 40060520 |
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| Ingar Romero A, Raicevic T, Al Boustani G, Gupta M, Heiler AC, Bichlmaier L, Barbone M, Becherer M, Kiriya D, Inoue S, Alexander J, Müller K, Bausch AR, Wolfrum B, Teshima TF, Self-Foldable Three-Dimensional Biointerfaces by Strain Engineering of Two-Dimensional Layered Materials on Polymers ACS applied materials & interfaces17:10305-10315 2025 |
| PubMed ID: 39879108 |
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| Jæger KH, Charwat V, Healy KE, Wall S, Tveito A, Determining properties of human-induced pluripotent stem cell-derived cardiomyocytes using spatially resolved electromechanical metrics The Journal of physiology17:10305-10315 2025 |
| PubMed ID: 39960634 |
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| Zhou Y, Su Y, Yang Q, Li J, Hong Y, Gao T, Zhong Y, Ma X, Jin M, Liu X, Yuan N, Kennedy BC, Wang L, Yan L, Viaene AN, Helbig I, Kessler SK, Kleinman JE, Hyde TM, Nauen DW, Liu C, Liu Z, Shen Z, Li C, Xu S, He J, Weinberger DR, Ming GL, Song H, Comparative molecular landscapes of immature neurons in the mammalian dentate gyrus across species reveal special features in humans bioRxiv : the preprint server for biology17:10305-10315 2025 |
| PubMed ID: 40027814 |
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| Alghadeer A, Patni AP, Li Z, Lim YC, Mathieu J, Ruohola-Baker H, Protocol for generating three-dimensional induced early ameloblasts using serum-free media and growth factors STAR protocols5:103100 2024 |
| PubMed ID: 38824640 |
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| Balashova OA, Panoutsopoulos AA, Visina O, Selhub J, Knoepfler PS, Borodinsky LN, Noncanonical function of folate through folate receptor 1 during neural tube formation Nature communications15:1642 2024 |
| PubMed ID: 38388461 |
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| Cao H, Yue L, Shao J, Kong F, Liu S, Huai H, He Z, Mao Z, Yang Y, Tan Y, Wang H, Small extracellular vesicles derived from umbilical cord mesenchymal stem cells alleviate radiation-induced cardiac organoid injury Stem cell research & therapy15:493 2024 |
| PubMed ID: 39707562 |
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| Dorion MF, Yaqubi M, Senkevich K, Kieran NW, MacDonald A, Chen CX, Luo W, Wallis A, Shlaifer I, Hall JA, Dudley RWR, Glass IA, Birth Defects Research Laboratory IA, Stratton JA, Fon E, Bartels T, Antel JP, Gan-Or Z, Durcan TM, Healy LM, MerTK is a mediator of alpha-synuclein fibril uptake by human microglia Brain : a journal of neurology15:493 2024 |
| PubMed ID: 37671615 |
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| Fatima N, Dillen L, Hommersom MP, Çepni E, Fatima F, van Beusekom E, Albert S, Ali Khan A, de Brouwer APM, van Bokhoven H, Generation of induced pluripotent stem cell line (UCSFi001-A-77) carrying a biallelic frameshift variant in exon 4 of SGIP1 through CRISPR/Cas9 Stem cell research80:103511 2024 |
| PubMed ID: 39098170 |
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| Frenz-Wiessner S, Fairley SD, Buser M, Goek I, Salewskij K, Jonsson G, Illig D, Zu Putlitz B, Petersheim D, Li Y, Chen PH, Kalauz M, Conca R, Sterr M, Geuder J, Mizoguchi Y, Megens RTA, Linder MI, Kotlarz D, Rudelius M, Penninger JM, Marr C, Klein C, Generation of complex bone marrow organoids from human induced pluripotent stem cells Nature methods80:103511 2024 |
| PubMed ID: 38374263 |
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| Guo J, Jiang H, Schuftan D, Moreno JD, Ramahdita G, Aryan L, Bhagavan D, Silva J, Huebsch N, Substrate mechanics unveil early structural and functional pathology in iPSC micro-tissue models of hypertrophic cardiomyopathy iScience27:109954 2024 |
| PubMed ID: 38827401 |
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| Hwang DG, Choi H, Yong U, Kim D, Kang W, Park SM, Jang J, Bioprinting-Assisted Tissue Assembly for Structural and Functional Modulation of Engineered Heart Tissue Mimicking Left Ventricular Myocardial Fiber Orientation Advanced materials (Deerfield Beach, Fla)27:e2400364 2024 |
| PubMed ID: 38717016 |
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| Juliar BA, Stanaway IB, Sano F, Fu H, Smith KD, Akilesh S, Scales SJ, El Saghir J, Bhatraju PK, Liu E, Yang J, Lin J, Eddy S, Kretzler M, Zheng Y, Himmelfarb J, Harder JL, Freedman BS, Interferon-? induces combined pyroptotic angiopathy and APOL1 expression in human kidney disease Cell reports43:114310 2024 |
| PubMed ID: 38838223 |
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| Karbassi E, Padgett R, Bertero A, Reinecke H, Klaiman JM, Yang X, Hauschka SD, Murry CE, Targeted CRISPR activation is functional in engineered human pluripotent stem cells but undergoes silencing after differentiation into cardiomyocytes and endothelium Cellular and molecular life sciences : CMLS81:95 2024 |
| PubMed ID: 38372898 |
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| Lim SW, Lee KI, Cui S, Fang X, Shin YJ, Lee H, Lee JY, Chung BH, Yang CW, Generation of green fluorescent protein reporter knock-in iPSC line at the 3'UTR region of the KLOTHO locus Stem cell research80:103499 2024 |
| PubMed ID: 39111000 |
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| Mathis D, Koch J, Koller S, Sauter K, Flück C, Uldry AC, Forny P, Froese DS, Laemmle A, Induced pluripotent stem cell-derived hepatocytes reveal TCA cycle disruption and the potential basis for triheptanoin treatment for malate dehydrogenase 2 deficiency Molecular genetics and metabolism reports39:101066 2024 |
| PubMed ID: 38425868 |
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| Parodi G, Zanini G, Collo L, Impollonia R, Cervetto C, Frega M, Chiappalone M, Martinoia S, In vitro electrophysiological drug testing on neuronal networks derived from human induced pluripotent stem cells Stem cell research & therapy15:433 2024 |
| PubMed ID: 39551784 |
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| Podlesny-Drabiniok A, Novikova G, Liu Y, Dunst J, Temizer R, Giannarelli C, Marro S, Kreslavsky T, Marcora E, Goate AM, BHLHE40/41 regulate microglia and peripheral macrophage responses associated with Alzheimer's disease and other disorders of lipid-rich tissues Nature communications15:2058 2024 |
| PubMed ID: 38448474 |
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| Prondzynski M, Berkson P, Trembley MA, Tharani Y, Shani K, Bortolin RH, Sweat ME, Mayourian J, Yucel D, Cordoves AM, Gabbin B, Hou C, Anyanwu NJ, Nawar F, Cotton J, Milosh J, Walker D, Zhang Y, Lu F, Liu X, Parker KK, Bezzerides VJ, Pu WT, Efficient and reproducible generation of human iPSC-derived cardiomyocytes and cardiac organoids in stirred suspension systems Nature communications15:5929 2024 |
| PubMed ID: 39009604 |
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| Prondzynski M, Bortolin RH, Berkson P, Trembley MA, Shani K, Sweat ME, Mayourian J, Cordoves AM, Anyanwu NJ, Tharani Y, Cotton J, Milosh JB, Walker D, Zhang Y, Liu F, Liu X, Parker KK, Bezzerides VJ, Pu WT, Efficient and reproducible generation of human iPSC-derived cardiomyocytes using a stirred bioreactor bioRxiv : the preprint server for biology15:5929 2024 |
| PubMed ID: 38464269 |
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| Qi L, Groeger M, Sharma A, Goswami I, Chen E, Zhong F, Ram A, Healy K, Hsiao EC, Willenbring H, Stahl A, Adipocyte inflammation is the primary driver of hepatic insulin resistance in a human iPSC-based microphysiological system Nature communications15:7991 2024 |
| PubMed ID: 39266553 |
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| Rahimov F, Nieminen P, Kumari P, Juuri E, Nikopensius T, Paraiso K, German J, Karvanen A, Kals M, Elnahas AG, Karjalainen J, Kurki M, Palotie A, FinnGen A, Estonian Biobank Research Team A, Heliövaara A, Esko T, Jukarainen S, Palta P, Ganna A, Patni AP, Mar D, Bomsztyk K, Mathieu J, Ruohola-Baker H, Visel A, Fakhouri WD, Schutte BC, Cornell RA, Rice DP, High incidence and geographic distribution of cleft palate in Finland are associated with the IRF6 gene Nature communications15:9568 2024 |
| PubMed ID: 39500877 |
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| Simmons DW, Malayath G, Schuftan DR, Guo J, Oguntuyo K, Ramahdita G, Sun Y, Jordan SD, Munsell MK, Kandalaft B, Pear M, Rentschler SL, Huebsch N, Engineered tissue geometry and Plakophilin-2 regulate electrophysiology of human iPSC-derived cardiomyocytes APL bioengineering8:016118 2024 |
| PubMed ID: 38476404 |
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| Sirbu A, Bathe-Peters M, Kumar JLM, Inoue A, Lohse MJ, Annibale P, Cell swelling enhances ligand-driven ß-adrenergic signaling Nature communications15:7822 2024 |
| PubMed ID: 39242606 |
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| van Sluis M, Yu Q, van der Woude M, Gonzalo-Hansen C, Dealy SC, Janssens RC, Somsen HB, Ramadhin AR, Dekkers DHW, Wienecke HL, Demmers JJPG, Raams A, Davó-Martínez C, Llerena Schiffmacher DA, van Toorn M, Häckes D, Thijssen KL, Zhou D, Lammers JG, Pines A, Vermeulen W, Pothof J, Demmers JAA, van den Berg DLC, Lans H, Marteijn JA, Transcription-coupled DNA-protein crosslink repair by CSB and CRL4 Nature cell biology15:7822 2024 |
| PubMed ID: 38600236 |
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| Voogd EJHF, Thijs M, Levers MR, Hofmeijer J, Frega M, Hypothermia improves neuronal network recovery in a human-derived in vitro model of oxygen-deprivation PloS one19:e0314913 2024 |
| PubMed ID: 39705243 |
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| Woo Lim S, In Lee K, Cui S, Fang X, Jin Shin Y, Lee H, Woo Yang C, Young Lee J, Ha Chung B, Generation of a human SLC12A3 knock-in human induced pluripotent stem cell line (CMCi014-A-82) using CRISPR-Cas9 system Stem cell research81:103522 2024 |
| PubMed ID: 39163808 |
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| Yang P, Zhu L, Wang S, Gong J, Selvaraj JN, Ye L, Chen H, Zhang Y, Wang G, Song W, Li Z, Cai L, Zhang H, Zhang D, Engineered model of heart tissue repair for exploring fibrotic processes and therapeutic interventions Nature communications15:7996 2024 |
| PubMed ID: 39266508 |
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| Zenk F, Fleck JS, Jansen SMJ, Kashanian B, Eisinger B, Santel M, Dupré JS, Camp JG, Treutlein B, Single-cell epigenomic reconstruction of developmental trajectories from pluripotency in human neural organoid systems Nature neuroscience15:7996 2024 |
| PubMed ID: 38914828 |
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| Dorion MF, Yaqubi M, Murdoch HJ, Hall JA, Dudley R, Antel JP, Durcan TM, Healy LM, Systematic comparison of culture media uncovers phenotypic shift of primary human microglia defined by reduced reliance to CSF1R signaling Glia71:1278-1293 2023 |
| PubMed ID: 36680780 |
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| Dyke E, Bijnagte-Schoenmaker C, Wu KM, Oudakker A, Roepman R, Nadif Kasri N, Generation of induced pluripotent stem cell line carrying frameshift variants in NPHP1 (UCSFi001-A-68) using CRISPR/Cas9 Stem cell research68:103053 2023 |
| PubMed ID: 36842376 |
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| Geben LC, Brockman AA, Chalkley MBL, Sweet SR, Gallagher JE, Scheuing AL, Simerly RB, Ess KC, Irish JM, Ihrie RA, Dephosphorylation of 4EBP1/2 Induces Prenatal Neural Stem Cell Quiescence bioRxiv : the preprint server for biology68:103053 2023 |
| PubMed ID: 36824760 |
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| Hasan S, Fernandopulle MS, Humble SW, Frankenfield AM, Li H, Prestil R, Johnson KR, Ryan BJ, Wade-Martins R, Ward ME, Hao L, Multi-modal Proteomic Characterization of Lysosomal Function and Proteostasis in Progranulin-Deficient Neurons bioRxiv : the preprint server for biology68:103053 2023 |
| PubMed ID: 36865171 |
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| Kaul H, Werschler N, Jones RD, Siu MM, Tewary M, Hagner A, Ostblom J, Aguilar-Hidalgo D, Zandstra PW, Virtual cells in a virtual microenvironment recapitulate early development-like patterns in human pluripotent stem cell colonies Stem cell reports18:377-393 2023 |
| PubMed ID: 36332630 |
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| Kornienko J, Rodríguez-Martínez M, Fenzl K, Hinze F, Schraivogel D, Grosch M, Tunaj B, Lindenhofer D, Schraft L, Kueblbeck M, Smith E, Mao C, Brown E, Owens A, Saguner AM, Meder B, Parikh V, Gotthardt M, Steinmetz LM, Mislocalization of pathogenic RBM20 variants in dilated cardiomyopathy is caused by loss-of-interaction with Transportin-3 Nature communications14:4312 2023 |
| PubMed ID: 37463913 |
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| Lavekar SS, Harkin J, Hernandez M, Gomes C, Patil S, Huang KC, Puntambekar SS, Lamb BT, Meyer JS, Development of a three-dimensional organoid model to explore early retinal phenotypes associated with Alzheimer's disease Scientific reports13:13827 2023 |
| PubMed ID: 37620502 |
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| Mijdam R, Bijnagte-Schoenmaker C, Dyke E, Moons SJ, Boltje TJ, Nadif Kasri N, Lefeber DJ, Sialic acid biosynthesis pathway blockade disturbs neuronal network formation in human iPSC-derived excitatory neurons Journal of neurochemistry167:76-89 2023 |
| PubMed ID: 37650222 |
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| Paranjape N, Lin YT, Flores-Ramirez Q, Sarin V, Johnson AB, Chu J, Paredes M, Wiita AP, A CRISPR-engineered isogenic model of the 22q112 A-B syndromic deletion Scientific reports13:7689 2023 |
| PubMed ID: 37169815 |
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| Parodi G, Brofiga M, Pastore VP, Chiappalone M, Martinoia S, Deepening the role of excitation/inhibition balance in human iPSCs-derived neuronal networks coupled to MEAs during long-term development Journal of neural engineering20:7689 2023 |
| PubMed ID: 37678214 |
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| Schimmel J, Muñoz-Subirana N, Kool H, van Schendel R, van der Vlies S, Kamp JA, de Vrij F, Kushner SA, Smith GCM, Boulton SJ, Tijsterman M, Modulating mutational outcomes and improving precise gene editing at CRISPR-Cas9-induced breaks by chemical inhibition of end-joining pathways Cell reports42:112019 2023 |
| PubMed ID: 36701230 |
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| Simmons DW, Schuftan DR, Ramahdita G, Huebsch N, Hydrogel-Assisted Double Molding Enables Rapid Replication of Stereolithographic 3D Prints for Engineered Tissue Design ACS applied materials & interfaces15:25313-25323 2023 |
| PubMed ID: 37200617 |
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| Wesley CC, Levy DL, Differentiation-dependent changes in lamin B1 dynamics and lamin B receptor localization Molecular biology of the cell34:ar10 2023 |
| PubMed ID: 36598800 |
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| Wu JY, Yeager K, Tavakol DN, Morsink M, Wang B, Soni RK, Hung CT, Vunjak-Novakovic G, Directed differentiation of human iPSCs into mesenchymal lineages by optogenetic control of TGF-ß signaling Cell reports42:112509 2023 |
| PubMed ID: 37178118 |
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| Youn J, Rhyou J, Kim D, Lee J, Choi JW, Park TE, Kim DS, Facile and adhesive-free method for bonding nanofiber membrane onto thermoplastic polystyrene substrate to fabricate 3D cell culture platforms Materials today Bio20:100648 2023 |
| PubMed ID: 37214546 |
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| Zanini G, Parodi G, Chiappalone M, Martinoia S, Investigating the reliability of the evoked response in human iPSCs-derived neuronal networks coupled to micro-electrode arrays APL bioengineering7:046121 2023 |
| PubMed ID: 38130601 |
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| Barnes AM, Holmstoen TB, Bonham AJ, Rowland TJ, Differentiating Human Pluripotent Stem Cells to Cardiomyocytes Using Purified Extracellular Matrix Proteins Bioengineering (Basel, Switzerland)9:046121 2022 |
| PubMed ID: 36550926 |
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| Bauer L, Rissmann M, Benavides FFW, Leijten L, van Run P, Begeman L, Veldhuis Kroeze EJB, Lendemeijer B, Smeenk H, de Vrij FMS, Kushner SA, Koopmans MPG, Rockx B, van Riel D, In vitro and in vivo differences in neurovirulence between D614G, Delta And Omicron BA.1 SARS-CoV-2 variants Acta Neuropathol Communications10:046121 2022 |
| PubMed ID: 36058935 |
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| Brown AL, Wilkins OG, Keuss MJ, Hill SE, Zanovello M, Lee WC, Bampton A, Lee FCY, Masino L, Qi YA, Bryce-Smith S, Gatt A, Hallegger M, Fagegaltier D, Phatnani H, NYGC ALS Consortium H, Newcombe J, Gustavsson EK, Seddighi S, Reyes JF, Coon SL, Ramos D, Schiavo G, Fisher EMC, Raj T, Secrier M, Lashley T, Ule J, Buratti E, Humphrey J, Ward ME, Fratta P, TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A Nature603:131-137 2022 |
| PubMed ID: 35197628 |
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| Budjan C, Liu S, Ranga A, Gayen S, Pourquié O, Hormoz S, Paraxial mesoderm organoids model development of human somites eLife11:131-137 2022 |
| PubMed ID: 35088712 |
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| Charwat V, Charrez B, Siemons BA, Finsberg H, Jæger KH, Edwards AG, Huebsch N, Wall S, Miller E, Tveito A, Healy KE, Validating the Arrhythmogenic Potential of High-, Intermediate-, and Low-Risk Drugs in a Human-Induced Pluripotent Stem Cell-Derived Cardiac Microphysiological System ACS pharmacology & translational science5:652-667 2022 |
| PubMed ID: 35983280 |
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| Dräger NM, Sattler SM, Huang CT, Teter OM, Leng K, Hashemi SH, Hong J, Aviles G, Clelland CD, Zhan L, Udeochu JC, Kodama L, Singleton AB, Nalls MA, Ichida J, Ward ME, Faghri F, Gan L, Kampmann M, A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states Nature neuroscience25:1149-1162 2022 |
| PubMed ID: 35953545 |
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| Fort L, Gama V, Macara IG, Stem cell conversion to the cardiac lineage requires nucleotide signalling from apoptosing cells Nature cell biology24:434-447 2022 |
| PubMed ID: 35414019 |
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| Gerosa L, Mazzoleni S, Rusconi F, Longaretti A, Lewerissa E, Pelucchi S, Murru L, Giannelli SG, Broccoli V, Marcello E, Kasri NN, Battaglioli E, Passafaro M, Bassani S, The epilepsy-associated protein PCDH19 undergoes NMDA receptor-dependent proteolytic cleavage and regulates the expression of immediate-early genes Cell reports39:110857 2022 |
| PubMed ID: 35613587 |
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| Goddi A, Carmona A, Park SY, Dalgin G, Gonzalez Porras MA, Brey EM, Cohen RN, Laminin-a4 Negatively Regulates Adipocyte Beiging Through the Suppression of AMPKa in Male Mice Endocrinology163:110857 2022 |
| PubMed ID: 36124842 |
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| He Z, Maynard A, Jain A, Gerber T, Petri R, Lin HC, Santel M, Ly K, Dupré JS, Sidow L, Sanchis Calleja F, Jansen SMJ, Riesenberg S, Camp JG, Treutlein B, Lineage recording in human cerebral organoids Nature methods19:90-99 2022 |
| PubMed ID: 34969984 |
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| Hommersom MP, Bijnagte-Schoenmaker C, Albert S, van de Warrenburg BPC, Nadif Kasri N, van Bokhoven H, Generation of induced pluripotent stem cell lines carrying monoallelic (UCSFi001-A-60) or biallelic (UCSFi001-A-61; UCSFi001-A-62) frameshift variants in CACNA1A using CRISPR/Cas9 Stem cell research61:102730 2022 |
| PubMed ID: 35286975 |
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| Khayrullina G, Alipio-Gloria ZA, Deguise MO, Gagnon S, Chehade L, Stinson M, Belous N, Bergman EM, Lischka FW, Rotty J, Dalgard CL, Kothary R, Johnson KA, Burnett BG, Survival motor neuron protein deficiency alters microglia reactivity Glia70:1337-1358 2022 |
| PubMed ID: 35373853 |
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| King O, Cruz-Moreira D, Sayed A, Kermani F, Kit-Anan W, Sunyovszki I, Wang BX, Downing B, Fourre J, Hachim D, Randi AM, Stevens MM, Rasponi M, Terracciano CM, Functional microvascularization of human myocardium Cell reports methods2:100280 2022 |
| PubMed ID: 36160044 |
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| Li SR, Gulieva RE, Helms L, Cruz NM, Vincent T, Fu H, Himmelfarb J, Freedman BS, Glucose absorption drives cystogenesis in a human organoid-on-chip model of polycystic kidney disease Nature communications13:7918 2022 |
| PubMed ID: 36564419 |
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| Mills C, Riching A, Keller A, Stombaugh J, Haupt A, Maksimova E, Dickerson SM, Anderson E, Hemphill K, Ebmeier C, Schiel JA, Levenga J, Perkett M, Smith AVB, Strezoska Z, A Novel CRISPR Interference Effector Enabling Functional Gene Characterization with Synthetic Guide RNAs The CRISPR journal5:769-786 2022 |
| PubMed ID: 36257604 |
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| Oguntuyo K, Schuftan D, Guo J, Simmons D, Bhagavan D, Moreno JD, Kang PW, Miller E, Silva JR, Huebsch N, Robust, Automated Analysis of Electrophysiology in Induced Pluripotent Stem Cell-Derived Micro-Heart Muscle for Drug Toxicity Tissue engineering Part C, Methods28:457-468 2022 |
| PubMed ID: 35925789 |
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| Schneider O, Moruzzi A, Fuchs S, Grobel A, Schulze HS, Mayr T, Loskill P, Fusing spheroids to aligned µ-tissues in a heart-on-chip featuring oxygen sensing and electrical pacing capabilities Materials today Bio15:100280 2022 |
| PubMed ID: 35601892 |
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| Williams LM, Fujimoto T, Weaver RR, Logsdon AF, Evitts KM, Young JE, Banks WA, Erickson MA, Prolonged culturing of iPSC-derived brain endothelial-like cells is associated with quiescence, downregulation of glycolysis, and resistance to disruption by an Alzheimer's brain milieu Fluids and barriers of the CNS19:10 2022 |
| PubMed ID: 35123529 |
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| Xu Y, Kuppe C, Perales-Patón J, Hayat S, Kranz J, Abdallah AT, Nagai J, Li Z, Peisker F, Saritas T, Halder M, Menzel S, Hoeft K, Kenter A, Kim H, van Roeyen CRC, Lehrke M, Moellmann J, Speer T, Buhl EM, Hoogenboezem R, Boor P, Jansen J, Knopp C, Kurth I, Smeets B, Bindels E, Reinders MEJ, Baan C, Gribnau J, Hoorn EJ, Steffens J, Huber TB, Costa I, Floege J, Schneider RK, Saez-Rodriguez J, Freedman BS, Kramann R, Adult human kidney organoids originate from CD24 Nature genetics54:1690-1701 2022 |
| PubMed ID: 36303074 |
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| Aisenbrey EA, Torr E, Johnson H, Soref C, Daly W, Murphy WL, A protocol for rapid pericyte differentiation of human induced pluripotent stem cells STAR protocols2:100261 2021 |
| PubMed ID: 33490977 |
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| Charrez B, Charwat V, Siemons BA, Goswami I, Sakolish C, Luo YS, Finsberg H, Edwards AG, Miller EW, Rusyn I, Healy KE, Heart Muscle Microphysiological System for Cardiac Liability Prediction of Repurposed COVID-19 Therapeutics Frontiers in pharmacology12:684252 2021 |
| PubMed ID: 34421592 |
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| Christopher GA, Noort RJ, Esseltine JL, Connexin 43 Gene Ablation Does Not Alter Human Pluripotent Stem Cell Germ Lineage Specification Biomolecules12:684252 2021 |
| PubMed ID: 35053163 |
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| Guo J, Jiang H, Oguntuyo K, Rios B, Boodram Z, Huebsch N, Interplay of Genotype and Substrate Stiffness in Driving the Hypertrophic Cardiomyopathy Phenotype in iPSC-Micro-Heart Muscle Arrays Cellular and molecular bioengineering14:409-425 2021 |
| PubMed ID: 34777601 |
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| Helms L, Marchiano S, Stanaway IB, Hsiang TY, Juliar BA, Saini S, Zhao YT, Khanna A, Menon R, Alakwaa F, Mikacenic C, Morrell ED, Wurfel MM, Kretzler M, Harder JL, Murry CE, Himmelfarb J, Ruohola-Baker H, Bhatraju PK, Gale M, Freedman BS, Cross-validation of SARS-CoV-2 responses in kidney organoids and clinical populations JCI insight6:409-425 2021 |
| PubMed ID: 34767537 |
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| Kuramochi Y, Awaya T, Matsuo-Takasaki M, Takami M, An Y, Li J, Hemmi Y, Wakabayashi T, Arai Y, Inoue J, Noguchi M, Nakamura Y, Asaka I, Akimoto K, Saito MK, Hayashi Y, Generation of two human induced pluripotent stem cell lines derived from two X-linked adrenoleukodystrophy patients with ABCD1 mutations Stem cell research53:102337 2021 |
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| Zinger A, Cvetkovic C, Sushnitha M, Naoi T, Baudo G, Anderson M, Shetty A, Basu N, Covello J, Tasciotti E, Amit M, Xie T, Taraballi F, Krencik R, Humanized Biomimetic Nanovesicles for Neuron Targeting Advanced science (Weinheim, Baden-Wurttemberg, Germany)57:e2101437 2021 |
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| Arai Y, Takami M, An Y, Matsuo-Takasaki M, Hemmi Y, Wakabayashi T, Inoue J, Noguchi M, Nakamura Y, Sugimoto K, Takemura T, Okita K, Osafune K, Takasato M, Hayata T, Hayashi Y, Generation of two human induced pluripotent stem cell lines derived from two juvenile nephronophthisis patients with NPHP1 deletion Stem cell research45:101815 2020 |
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| Lim ET, Chan Y, Dawes P, Guo X, Erdin S, Tai DJC, Liu S, Reichert JM, Burns MJ, Chan YK, Chiang JJ, Meyer K, Zhang X, Walsh CA, Yankner BA, Raychaudhuri S, Hirschhorn JN, Gusella JF, Talkowski ME, Church GM, Orgo-Seq integrates single-cell and bulk transcriptomic data to identify cell type specific-driver genes associated with autism spectrum disorder Nature communications13:3243 2020 |
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| Zampeta IF, Sonzogni M, Niggl E, Lendemeijer B, Smeenk H, de Vrij FMS, Kushner SA, Distel B, Elgersma Y, Conserved UBE3A subcellular distribution between human and mice is facilitated by non-homologous isoforms Human molecular genetics12:6167 2020 |
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| Passage Frozen |
40 |
| Split Ratio |
1:8 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
mTeSR1 |
| Serum |
none |
| Substrate |
Matrigel |
| Supplement |
- |
|