GM50069
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 18 SOMATIC CELL HYBRIDS
Repository
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NIGMS Human Genetic Cell Repository
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Subcollection |
Hybrids |
Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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Human/hamster somatic cell hybrid retaining a human 18pter>18q11.2 translocated to a human chromosome and as der(18)(pter>q11.2::q11.2>pter) in 100% of cells and other human chromosomes
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Remarks
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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Remarks |
Line JH211; produced by the fusion of human lymphoblasts from a patient with trisomy 18p syndrome [47,XX,+del(18)(q11.2)] with Chinese hamster line UCW56 cells; grown in culture medium with 0.2mM proline at 39C; this hybrid retains a human 18pter>18q11.2 translocated to a human chromosome and as der(18)(pter>q11.2::q11.2>pter) in 100% of cells along with other human chromosomes |
Kline AD, Rojas K, Mewar R, Moshinsky D, Overhauser J, Somatic cell hybrid deletion map of human chromosome 18. Genomics13:1-6 1992 |
PubMed ID: 1577474 |
Split Ratio |
1:8 |
Temperature |
39 C |
Percent CO2 |
10% |
Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Proline 0.2 mM |
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