GM50086
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 18 SOMATIC CELL HYBRIDS
Repository
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NIGMS Human Genetic Cell Repository
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Subcollection |
Hybrids |
Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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Human/hamster somatic cell hybrid retaining a human del(18)(p11.1) in 100% of cells and human chromosomes 3, 4, 5, 7, 8, 9, 11, 12, 13, 14, 15, 16, 17, 20, 21, and Y
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Remarks
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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Remarks |
Line JH410; produced by the fusion of human lymphoblasts from a patient with 18p- syndrome with karyotype del(18)(p11.1), with a Chinese hamster line; grown at 39C; retains a human del(18)(p11.1) in 100% of cells and other human chromosomes |
Split Ratio |
1:7 |
Temperature |
39 C |
Percent CO2 |
8% |
Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
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