NA11691
DNA from Somatic cell hybrid
Description:
CHROMOSOME 8 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
| Quantity |
0.050mg |
| Quantitation Method |
Please see our FAQ |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Sample Source
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DNA from Somatic cell hybrid
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Chinese hamster/human hybrid retaining human 8pter>8cen
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 8 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 8q22q23, D8S5, and 8q13q22.1, CA2, and gave a positive result with a probe for 8p21, NEFL. M. Wagner, Research Triangle Park, NC, (personal communication) reported that PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 8p22, D8S258, and 8p21.3-cen, D8S259, and gave negative results with primers for 8q11, MOS, 8q13q22.1, D8S286, 8q22.2, D8S271, 8q22.3, D8S276, and 8q24.3-qter, D8S263. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 8p12, D8S87, 8p21-p11.2, LHRH, and 8p23-p22, and gave negative results with primers for 8q11.22-q12, D8S260, 8q22.2, D8S270, 8q23-q24, D8S199, and 8q24, TG. |
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| Remarks |
Subclone 8 of line 1HL35; produced by the fusion of normal human lymphos with Chinese hamster ovary irs-1 cells; grown in medium without selection; retains a fragment of human chromosome #8 extending from 8pter>8cen in 100% of cells |
| White RA, Dowler LL, Pasztor LM, Gatson LL, Adkison LR, Angeloni SV, Wilson DB, Assignment of the transcription factor GATA4 gene to human chromosome 8 and mouse chromosome 14: Gata4 is a candidate gene for Ds (disorganization). Genomics27:20-6 1995 |
| PubMed ID: 7665171 |
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| Wagner MJ, Ge Y, Siciliano M, Wells DE, A hybrid cell mapping panel for regional localization of probes to human chromosome 8. Genomics10:114-25 1991 |
| PubMed ID: 2045096 |
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