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NA11754 DNA from Somatic cell hybrid

Description:

CHROMOSOME 3 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Hybrids
Quantity 0.050mg
Quantitation Method Please see our FAQ
Cell Type Somatic cell hybrid
Transformant Untransformed
Sample Source DNA from Somatic cell hybrid
Confirmation Clinical summary/Case history
ISCN Chinese hamster/human hybrid retaining human 3pter>3q25 translocated to a hamster chromosome
Remarks Line R227-3A; human cells fused with CHO Urd-C cells; selected for loss of human transferrin receptor; grown in uridinedef F12 medium with 5% dialyzed FBS; retains human 3pter>3q25 translocated to a hamster chromosome in 96% of cells

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 3p14.1-p12, D3S1566, 3p22-p21.33, GLB1, 3p25p24.2, D3S1304, 3q13.1-q13.2, D3S1310, and 3q22-q24, D3S1764, and gave negative results with primers for 3q26.2-q27, GLUT2, and 3q28-q29, D3S1311.
 

Phenotypic Data

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Remarks Line R227-3A; human cells fused with CHO Urd-C cells; selected for loss of human transferrin receptor; grown in uridinedef F12 medium with 5% dialyzed FBS; retains human 3pter>3q25 translocated to a hamster chromosome in 96% of cells

Publications

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Ayyanathan K, Naylor SL, Kunapuli SP, Structural characterization and fine chromosomal mapping of the human P2Y1 purinergic receptor gene (P2RY1). Somat Cell Mol Genet22:419-24 1996
PubMed ID: 9039850
 
Bailey A, Norris AL, Leek JP, Clissold PM, Carr IM, Ogilvie DJ, Morrison JF, Meredith DM, Markham AF, Yeast artificial chromosome cloning of the beta-catenin locus on human chromosome 3p21-22. Chromosome Res3:201-3 1995
PubMed ID: 7780664
 
Hess JF, Casselman JT, FitzGerald PG, Chromosomal locations of the genes for the beaded filament proteins CP 115 and CP 47. Curr Eye Res14:11-8 1995
PubMed ID: 7720401
 
Lin Y, Sheridan PL, Jones KA, Evans GA, The HIP116 SNF2/SW12-related transcription factor gene (SNF2L3) is located on human chromosome 3q25.1-q26.1. Genomics27:381-2 1995
PubMed ID: 7558014
 
Naylor SL, Buys CH, Carritt B, Report and abstracts of the Fourth International Workshop on Human Chromosome 3 Mapping Cytogenetics and cell genetics65:2-50 1994
PubMed ID: 8104766
 
Drabkin H, Wright M, Jonsen M, Varkony T, Jones C, Sage M, Gold S, Morse H, Mendez M, Erickson P, Development of a somatic cell hybrid mapping panel and molecular probes for human chromosome 3. Genomics8:435-46 1990
PubMed ID: 2286371

External Links

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dbSNP dbSNP ID: 11636
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International/Commercial/For-profit:
$449.00USD
U.S. Academic/Non-profit/Government:
$263.00USD
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