NA11941
DNA from Somatic cell hybrid
Description:
ADDITIONAL HUMAN/MOUSE SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
| Quantity |
0.050mg |
| Quantitation Method |
Please see our FAQ |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Sample Source
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DNA from Somatic cell hybrid
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Family Member
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11
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/mouse hybrid retaining a human chromo #11 & Xp translocated to a mouse chromosome
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for Xp22.32 and Yp11.3, MIC2, and gave a negative result with a probe for Xq28, DXS15. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp11.3, DXS1003, Xp22.32, STS, and Xq12, DXS453, and gave negative results with primers for Xq13.3, PGK1, Xq21.3, DXS3, Xq26, HPRT, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 11p13, CAT, and 11q23.3, ETS1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 12 |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 12p13.2, PRB1, and 12q14q24.1, D12S7. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17p13.1, MYH2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 22q12.3q13.1, PDGFB. |
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| Remarks |
Line 1W1LA4.9; produced by the fusion of human lymphocytes, 46,XY, with HPRT-def mouse L cell line 1R cells; grown in medium without selection; retains human chromosome #11 in 100% of cells & Xp translocated to a mouse chromo in 96% |
| Halsall JA, Andrews S, Krueger F, Rutledge CE, Ficz G, Reik W, Turner BM, Histone modifications form a cell-type-specific chromosomal bar code that persists through the cell cycle Scientific reports11:3009 2020 |
| PubMed ID: 33542322 |
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| Prawitt D, Enklaar T, Klemm G, Gartner B, Spangenberg C, Winterpacht A,
Higgins M, Pelletier J, Zabel B, Identification and characterization of MTR1, a novel gene with homology to
melastatin (MLSN1) and the trp gene family located in the BWS-WT2 critical
region on chromosome 11p15.5 and showing allele-specific expression. Curr Biol9(2):203-16 2000 |
| PubMed ID: 10607831 |
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| Gabriel JM, Higgins MJ, Gebuhr TC, Shows TB, Saitoh S, Nicholls RD, A model system to study genomic imprinting of human genes. Proc Natl Acad Sci U S A95:14857-62 1998 |
| PubMed ID: 9843980 |
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| Trask BJ, Friedman C, Martin-Gallardo A, Rowen L, Akinbami C, Blankenship J, Collins C, Giorgi D, Iadonato S, Johnson F, Kuo WL, Massa H, Morrish T, Naylor S, Nguyen OT, Rouquier S, Smith T, Wong DJ, Youngblom J, van den Engh G, Members of the olfactory receptor gene family are contained in large blocks of DNA duplicated polymorphically near the ends of human chromosomes. Hum Mol Genet7:13-26 1998 |
| PubMed ID: 9384599 |
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| Junien C, van Heyningen V, Evans G, Little P, Mannens M, Report of the second chromosome 11 workshop. Genomics12:620-5 1992 |
| PubMed ID: 1348491 |
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| Bickmore W, Christie S, van Heyningen V, Hastie ND, Porteous DJ, Hitch-hiking from HRAS1 to the WAGR locus with CMGT markers. Nucleic Acids Res16:51-60 1988 |
| PubMed ID: 2829125 |
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| Seawright A, Fletcher JM, Fantes JA, Morrison H, Porteous DJ, Li SS, Hastie ND, Van Heyningen V, Analysis of WAGR deletions and related translocations with gene- specific DNA probes, using FACS-selected cell hybrids. Somat Cell Mol Genet14:21-30 1988 |
| PubMed ID: 2829363 |
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| Spurr NK, Gough AC, Gosden J, Rout D, Porteous DJ, van Heyningen V, Docherty AJ, Restriction fragment length polymorphism analysis and assignment of the metalloproteinases stromelysin and collagenase to the long arm of chromosome 11. Genomics2:119-27 1988 |
| PubMed ID: 2900807 |
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| Hope RM, Goodfellow PN, Solomon E, Bodmer WF, Identification of MIC5, a human X-linked gene controlling expression of a cell surface antigen: definition by a monoclonal antibody raised against a human X mouse somatic cell hybrid. Cytogenet Cell Genet33:204-12 1982 |
| PubMed ID: 6889950 |
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| Nabholz M, Miggiano V, Bodmer W, Genetic analysis with human--mouse somatic cell hybrids. Nature223:358-63 1969 |
| PubMed ID: 4309885 |
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