NG05311
DNA from Fibroblast
Description:
SAIMIRI
ORDER: PRIMATES
Repository
|
NIA Aging Cell Culture Repository
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Permit Requirements
|
CITES PERMIT REQUIRED IF SHIPPING OUTSIDE OF USA
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Subcollection |
Nonhuman Primates Animals |
Quantity |
10 µg |
Quantitation Method |
Please see our FAQ |
Biopsy Source
|
Thigh
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
Species
|
Saimiri sciureus
|
Common Name
|
common squirrel monkey
|
Remarks
|
|
PDL at Freeze |
19 |
Passage Frozen |
14 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
|
Genbank |
COX1 |
Remark |
The culture was initiated from explants of minced skin tissue. Cell morphology is fibroblast-like. The karyotype is 44,XX; normal diploid female. The animal's age is not known. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
Diehl WE, Stansell E, Kaiser SM, Emerman M, Hunter E, Identification of Post-entry Restrictions to Mason-Pfizer Monkey Virus Infection in New World Monkey Cells Journal of virology82(22):11140-50 2008 |
PubMed ID: 18799582 |
|
Hughes JF, Coffin JM, Human endogenous retroviral elements as indicators of ectopic recombination events in the primate genome Genetics171:1183-94 2005 |
PubMed ID: 16157677 |
|
Gonzalez IL, Barth syndrome: TAZ gene mutations, mRNAs, and evolution. Am J Med Genet A134(4):409-14 2005 |
PubMed ID: 15793838 |
|
Steinert S, White DM, Zou Y, Shay JW, Wright WE, Telomere biology and cellular aging in nonhuman primate cells. Exp Cell Res272(2):146-52 2002 |
PubMed ID: 11777339 |
|
Kenyon L,Moraes C, Expanding the functional human mitochondrial DNA database by the establishment of primate xenomitochondrial cybrids. Proc Natl Acad Sci U S A94:9131-5 1997 |
PubMed ID: 9256447 |
|
Schempp W, Weber B, Muller G, Mammalian sex-chromosome evolution: a conserved homoeologous segment on the X and Y chromosomes in primates. Cytogenet Cell Genet50:201-5 1989 |
PubMed ID: 2805817 |
|
Nakamura KD, Hart RW, Comparison of proto oncogene expression in seven primate fibroblast cultures. Mech Ageing Dev39:177-87 1987 |
PubMed ID: 3626640 |
Cumulative PDL at Freeze |
19 |
Passage Frozen |
14 |
Split Ratio |
1:4 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
3% |
Medium |
MEM (Eagle) Alpha Modification with nucleosides/Dulbecco Modified Eagles Medium (high glucose), 1:1 mixture with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
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