NG05311
DNA from Fibroblast
Description:
SAIMIRI
ORDER: PRIMATES
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Repository
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NIA Aging Cell Culture Repository
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Permit Requirements
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CITES PERMIT REQUIRED IF SHIPPING OUTSIDE OF USA
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| Subcollection |
Nonhuman Primates
Animals |
| Quantity |
10 µg |
| Quantitation Method |
Please see our FAQ |
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Biopsy Source
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Thigh
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Saimiri sciureus
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Common Name
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common squirrel monkey
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Remarks
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| PDL at Freeze |
19 |
| Passage Frozen |
14 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Genbank |
COX1 |
| Remark |
The culture was initiated from explants of minced skin tissue. Cell morphology is fibroblast-like. The karyotype is 44,XX; normal diploid female. The animal's age is not known. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Diehl WE, Stansell E, Kaiser SM, Emerman M, Hunter E, Identification of Post-entry Restrictions to Mason-Pfizer Monkey Virus Infection in New World Monkey Cells Journal of virology82(22):11140-50 2008 |
| PubMed ID: 18799582 |
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| Hughes JF, Coffin JM, Human endogenous retroviral elements as indicators of ectopic recombination events in the primate genome Genetics171:1183-94 2005 |
| PubMed ID: 16157677 |
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| Gonzalez IL, Barth syndrome: TAZ gene mutations, mRNAs, and evolution. Am J Med Genet A134(4):409-14 2005 |
| PubMed ID: 15793838 |
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| Steinert S, White DM, Zou Y, Shay JW, Wright WE, Telomere biology and cellular aging in nonhuman primate cells. Exp Cell Res272(2):146-52 2002 |
| PubMed ID: 11777339 |
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| Kenyon L,Moraes C, Expanding the functional human mitochondrial DNA database by the establishment of primate xenomitochondrial cybrids. Proc Natl Acad Sci U S A94:9131-5 1997 |
| PubMed ID: 9256447 |
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| Schempp W, Weber B, Muller G, Mammalian sex-chromosome evolution: a conserved homoeologous segment on the X and Y chromosomes in primates. Cytogenet Cell Genet50:201-5 1989 |
| PubMed ID: 2805817 |
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| Nakamura KD, Hart RW, Comparison of proto oncogene expression in seven primate fibroblast cultures. Mech Ageing Dev39:177-87 1987 |
| PubMed ID: 3626640 |
| Cumulative PDL at Freeze |
19 |
| Passage Frozen |
14 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
3% |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides/Dulbecco Modified Eagles Medium (high glucose), 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
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