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CH01137 Neuronally-derived from Brain striatum

Description:

ST14A HTT-Q120, 1-548, HUMAN

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • Culture Protocols

Overview

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Repository HD Community Biorepository
Biopsy Source Brain striatum
Cell Type Neuronally-derived
Tissue Type Brain
Sample Source Neuronally-derived from Brain striatum
Family History N
Species Rattus norvegicus
Common Name brown or Norway rat
Remarks striatal cell line derived from rat embryonic day 14 (E14) expressing a human Huntingtin fragment (residues 1-548) containing 120 polyglutamine repeats

Characterizations

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Remarks striatal cell line derived from rat embryonic day 14 (E14) expressing a human Huntingtin fragment (residues 1-548) containing 120 polyglutamine repeats
Passage Frozen 2
 

Phenotypic Data

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Remarks striatal cell line derived from rat embryonic day 14 (E14) expressing a human Huntingtin fragment (residues 1-548) containing 120 polyglutamine repeats

Publications

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Cattaneo E, Conti L, Generation and characterization of embryonic striatal conditionally immortalized ST14A cells Journal of neuroscience research53:223-34 1998
PubMed ID: 9671979

Culture Protocols

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striatal cell line derived from rat embryonic day 14 (E14) expressing a human Huntingtin fragment (residues 1-548) containing 120 polyglutamine repeats
Passage Frozen 2
Split Ratio 1:4
Temperature 33 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent
Serum 10% fetal bovine serum Heat Inactivated
Substrate None specified
Remarks striatal cell line derived from rat embryonic day 14 (E14) expressing a human Huntingtin fragment (residues 1-548) containing 120 polyglutamine repeats
PDF CH01137 Cell Culturing Protocols
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$100.00USD
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