To facilitate research on differentiated cell types and other biomedical investigations, the Aging Cell Repository offers human induced pluripotent stem cell (hiPSC) lines.
In addition to QC tests (viability, contamination, karyotyping, microarray genotyping, and microsatellite matches with the parent cell culture), extensive pluripotency characterizations are performed on each NIA Repository hiPSC line. Pluripotency characterizations include the following assays:
- Surface antigen expression
- Embryoid body formation
- PluriTest gene expression assay
A complete Certificate of Analysis (CofA) that includes images and quantitative test results is available for each hiPSC line. The CofA PDF specifies which pluripotency characterizations were performed on each hiPSC line.
The Protocol PDFs outline the proper steps for successful handling of the hiPSCs. Additional information is also found on the CofA PDF, including necessary media components, substrates, passage methods, and split ratios.
* For commercial purposes, please note that Limited Use Label Licenses (LULLs) may apply.
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