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| Cell Line ID | Protein | Gene Name (gene symbol) | Tagged Alleles | Structure | Fluorescent Tag | Tag Location | Clone | Passage | Parental Line |
| AICS‑0005 | Paxillin | Paxillin (PXN) | Mono | Matrix adhesions | EGFP | C-terminus | 50 | 26 | GM25256 |
| AICS‑0010 | Sec61 beta | Sec61 translocon beta subunit (SEC61B) | Mono | Endoplasmic reticulum | mEGFP | N-terminus | 55 | 23 | GM25256 |
| AICS‑0011 | Tom20 | Translocase of outer mitrochondrial membrane 20 (TOMM20) | Mono | Mitochondria | mEGFP | C-terminus | 27 | 35 | GM25256 |
| AICS‑0012 | Alpha-tubulin | Tubulin-alpha 1b (TUBA1B) | Mono | Microtubules | mEGFP | N-terminus | 105 | 32 | GM25256 |
| AICS‑0013 | lamin B1 | Lamin B1 (LMNB1) | Mono | nuclear envelope | mEGFP | N-terminus | 210 | 30 | GM25256 |
| AICS‑0014 | Fibrillarin | Fibrillarin (FBL) | Mono | Nucleolus (Dense Fibrillar Component) | mEGFP | C-terminus | 6 | 25 | GM25256 |
| AICS‑0016 | Beta-actin | Actin beta (ACTB) | Mono | Actin filaments | mEGFP | N-terminus | 184 | 37 | GM25256 |
| AICS‑0017 | Desmoplakin | Desmoplakin (DSP) | Mono | Desmosomes | mEGFP | C-terminus | 65 | 31 | GM25256 |
| AICS‑0022‑037 | LAMP-1 | Lysosomal associated membrane protein mono 1 (LAMP1) | Mono | lysosomes | mEGFP | C-terminus | 37 | 22 | GM25256 |
| AICS‑0023 | Tight junction protein ZO-1 | Tight junction protein 1 (TJP1) | Mono | Tight junctions | mEGFP | N-terminus | 20 | 28 | GM25256 |
| AICS‑0024 | Non-muscle myosin heavy chain IIB | Myosin heavy chain 10 (MYH10) | Mono | Actomyosin bundles | mEGFP | N-terminus | 80 | 28 | GM25256 |
| AICS‑0025‑001 | Sialyltransferase 1 | ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1) | Mono | Golgi apparatus | mEGFP | C-terminus | 1 | 35 | GM25256 |
| AICS‑0025‑044 | Sialyltransferase 1 | ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1) | Bi | Golgi apparatus | mEGFP | C-terminus | 44 | 30 | GM25256 |
| AICS‑0030‑022 | Autophagy-related protein LC3 B | Microtubule-associated protein 1, light chain 3 beta (MAP1LC3B) | Mono | Autophagosomes | mEGFP | N-terminus | 22 | 28 | GM25256 |
| AICS‑0031‑035 | alpha-tubulin | Tubulin-alpha 1b (TUBA1B) | Mono | Microtubules | mTagRFP-T | N-terminus | 35 | 24 | GM25256 |
| AICS‑0032‑019 | Centrin-2 | Centrin 2 (CETN2) | Mono | Centrioles | mTagRFP-T | N-terminus | 19 | 22 | GM25256 |
| AICS‑0033‑115 | Peroxisomal membrane protein PMP34 | Solute carrier family 25 (mitochondrial carrier) member 17 (SLC25A17) | Mono | Peroxisomes | mEGFP | C-terminus | 115 | 28 | GM25256 |
| AICS‑0034‑062 | Lamin B1 | Lamin B1 (LMNB1) | Mono | Nuclear envelope | mTagRFP-T | N-terminus | 62 | 30 | GM25256 |
| AICS‑0036‑006 | NA | Safe harbor locus (AAVS1) | Mono | Cytoplasm | mEGFP | NA | 6 | 28 | GM25256 |
| AICS‑0036‑028 | NA | Safe harbor locus (AAVS1) | Mono | Cytoplasm | mEGFP | NA | 28 | 28 | GM25256 |
| AICS‑0037‑172 | Troponin I, slow skeletal type | Troponin I1, slow skeletal type (TNNI1) | Mono | Sarcomeric thin filaments | mEGFP | C-terminus | 172 | 34 | GM25256 |
| AICS‑0040‑013 | Ras-related protein Rab-5A | RAS-related protein RAB-5A (RAB5A) | Mono | Endosomes | mEGFP | N-terminus | 13 | 22 | GM25256 |
| AICS‑0040‑035 | Ras-related protein Rab-5A | RAS-related protein RAB-5A (RAB5A) | Bi | Endosomes | mEGFP | N-terminus | 35 | 22 | GM25256 |
| AICS‑0046‑051 | SERCA2 | ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 (ATP2A2) | Mono | Sarcoplasmic reticulum/Endoplasmic reticulum | mEGFP | N-terminus | 51 | 27 | GM25256 |
| AICS‑0048‑039 | Titin | Titin (TTN) | Mono | Sarcomere (M-line tag) | mEGFP | C-terminus | 39 | 35 | GM25256 |
| AICS‑0052‑003 | MLC-2a | Myosin light chain 7 (MYL7) | Mono | Sarcomeric thick filaments | mEGFP | C-terminus | 3 | 39 | GM25256 |
| AICS‑0053‑016 | Connexin-43 | Gap junction protein alpha 1 (GJA1) | Mono | Gap junctions | mEGFP | C-terminus | 16 | 25 | GM25256 |
| AICS‑0054‑091 | CAAX domain of K-Ras | Safe harbor locus (AAVS1) | Mono | Plasma membrane | mTagRFP-T | NA | 91 | 26 | GM25256 |
| AICS‑0057‑050 | Nucleophosmin | Nucleophosmin (NPM1) | Mono | Nucleolus (Granular Component) | mEGFP | C-terminus | 50 | 28 | GM25256 |
| AICS‑0058‑067 | beta-catenin | Catenin beta 1 (CTNNB1) | Mono | adherens junctions | mEGFP | N-terminus | 67 | 27 | GM25256 |