AG03204
Fibroblast from Lung, Lung
Description:
IMR-90 - SV40 VIRUS-TRANSFORMED FETAL LUNG FIBROBLAST
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Specially Characterized Fibroblasts |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Simian Virus 40
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Sample Source
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Fibroblast from Lung, Lung
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
47 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
DNA fingerprinting and HLA DQ-alpha typing showed that this pre-crisis SV40 transformed fibroblast culture had the same pattern and genotype as IMR90, the untransformed fibroblast. Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
AG03204A is a pre-crisis SV40-transformed IMR-90 culture. It was derived from the same pool of infected cells that initiated the post-crisis transformed IMR-90, AG02804C. Both cultures were initiated by infecting IMR-90 at PDL 30 with SV40 virus strain Rh 911. AG03204A contains 10% T-antigen positive cells and a low frequency of V-antigen positive cells. The morphology of the cells is a mixture of fibroblast-like and epithelial-like. |
| Schaefer DI, Livanos EM, White AE, Tlsty TD, Multiple mechanisms of N-(phosphonoacetyl)-L-aspartate drug resistance in SV40-infected precrisis human fibroblasts. Cancer Res53:4946-51 1993 |
| PubMed ID: 8402685 |
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| McNearney T, Ballard L, Seya T, Atkinson JP, Membrane cofactor protein of complement is present on human fibroblast, epithelial, and endothelial cells. J Clin Invest84:538-45 1989 |
| PubMed ID: 2474570 |
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| Hara H, Kaji H, Random integration of SV40 in SV40-transformed, immortalized human fibroblasts. Exp Cell Res168:531-8 1987 |
| PubMed ID: 3026824 |
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| Pettenati MJ, Le Beau MM, Lemons RS, Shima EA, Kawasaki ES, Larson RA, Sherr CJ, Diaz MO, Rowley JD, Assignment of CSF-1 to 5q33.1: evidence for clustering of genes regulating hematopoiesis and for their involvement in the deletion of the long arm of chromosome 5 in myeloid disorders. Proc Natl Acad Sci U S A84:2970-4 1987 |
| PubMed ID: 3495006 |
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| Stein GH, SV40-transformed human fibroblasts: evidence for cellular aging in pre- crisis cells. J Cell Physiol125:36-44 1985 |
| PubMed ID: 2995426 |
| Cumulative PDL at Freeze |
47 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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