AG04064
Fibroblast from Skin, Arm
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
GRC |
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Biopsy Source
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Arm
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
4.25 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like and the karyotype is 46,XY; diploid male. A culture initiated from a biopsy taken 2 years later from this same donor is AG08433B. Donor is the father of AG05807A and the grandfather of AG05838B, AG06962, and AG13003. Donor's cousin is AG13099. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19: 2018 |
| PubMed ID: 30567591 |
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| Kriete A, Mayo KL, Yalamanchili N, Beggs W, Bender P, Kari C, Rodeck U, Cell autonomous expression of inflammatory genes in biologically aged fibroblasts associated with elevated NF-kappaB activity Immunity & ageing : I & A5:5 2008 |
| PubMed ID: 18631391 |
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| Reed MJ, Bradshaw AD, Shaw M, Sadoun E, Han N, Ferara N, Funk S, Puolakkainen P, Sage EH, Enhanced angiogenesis characteristic of SPARC-null mice disappears with age Journal of cellular physiology204:800-7 2005 |
| PubMed ID: 15795937 |
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| Geigl JB, Langer S, Barwisch S, Pfleghaar K, Lederer G, Speicher MR, Analysis of gene expression patterns and chromosomal changes associated with aging. Cancer Res64(23):8550-7 2004 |
| PubMed ID: 15574761 |
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| Ly DH, Lockhart DJ, Lerner RA, Schultz PG, Mitotic misregulation and human aging. Science287(5462):2486-92 2000 |
| PubMed ID: 10741968 |
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| Cristofalo VJ, Allen RG, Pignolo RJ, Martin BG, Beck JC, Relationship between donor age and the replicative lifespan of human cells in culture: a reevaluation. Proc Natl Acad Sci U S A95:10614-9 1998 |
| PubMed ID: 9724752 |
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| Reed, TGF-beta 1 induces the expression of type 1 collagen and SPARC, and enhances contraction of collagen gels, by fibroblasts from young and aged donors. J Cell Biol158:169 (1994):10614-9 1994 |
| PubMed ID: 9724752 |
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| Guernsey DL, Koebbe M, Thomas JE, Myerly TK, Zmolek D, An altered response in the induction of cell membrane (Na + K)ATPase by thyroid hormone is characteristic of senescence in cultured human fibroblasts. Mech Ageing Dev33:283-93 1986 |
| PubMed ID: 3012221 |
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| Schneider EL, Monticone R, Smith J, Braunschweiger K, Roberts T, Skin fibroblast cultures derived from members of the Baltimore longitudinal study: a new resource for studies of cellular aging. Cytogenet Cell Genet31:40-6 1981 |
| PubMed ID: 7307581 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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