AG04454
Fibroblast from Skin, Arm
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
GRC |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY,der(15)(15qter>15p11.2::17q21>17qter)[34]/45,X[3]/46,XY,t(2;10)(2qter>p23::10p13>10pter;10qter>10p13::2p23>2pter)[2]/46,XY[11]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
28 |
| PDL at Freeze |
19 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like. The culture is a mosaic with karyotype: 46,XY/46,XY,Xq-,10q+,t(9;17)(p13;q23)/46,XY,t(1;18)(p34; p11.2); balanced; 56%/32%/7% with 5% of the cells examined showing random chromosomal abnormalities. A culture initiated from a biopsy taken 16 years later from this same donor is AG13076. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Greco M, Villani G, Mazzucchelli F, Bresolin N, Papa S, Attardi G, Marked aging-related decline in efficiency of oxidative phosphorylation in human skin fibroblasts The FASEB journal : official publication of the Federation of American Societies for Experimental Biology17:1706-8 2003 |
| PubMed ID: 12958183 |
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| Zhang J, Asin-Cayuela J, Fish J, Michikawa Y, Bonafe M, Olivieri F, Passarino G, De Benedictis G, Franceschi C, Attardi G, Strikingly higher frequency in centenarians and twins of mtDNA mutation causing remodeling of replication origin in leukocytes. Proc Natl Acad Sci U S A100(3):1116-21 2003 |
| PubMed ID: 12538859 |
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| Schneider EL, Monticone R, Smith J, Braunschweiger K, Roberts T, Skin fibroblast cultures derived from members of the Baltimore longitudinal study: a new resource for studies of cellular aging. Cytogenet Cell Genet31:40-6 1981 |
| PubMed ID: 7307581 |
| PDL at Senescence |
28 |
| Cumulative PDL at Freeze |
19.26 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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