AG04676
Epithelial from Eye
Description:
ORYCTOLAGUS
ORDER: LAGOMORPHA
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Animals |
|
Biopsy Source
|
Eye
|
|
Cell Type
|
Epithelial
|
|
Tissue Type
|
Lens
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Epithelial from Eye
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
44,XX
|
|
Species
|
Oryctolagus cuniculus
|
|
Common Name
|
rabbit
|
|
Remarks
|
|
| PDL at Freeze |
112 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
The culture was initiated on 9/2/79 from the ocular lens capsule of a New Zealand White rabbit. Cell morphology is epithelial-like. The karyotype is 44,XX; normal diploid female. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Hofmann W, Schubert D, LaBonte J, Munson L, Gibson S, Scammell J, Ferrigno P, Sodroski J, Species-specific, postentry barriers to primate immunodeficiency virus infection. J Virol73:10020-8 1999 |
| PubMed ID: 10559316 |
| |
| Megraud F, Gamon LB, McGarrity GJ, Characterization of Spiroplasma mirum (suckling mouse cataract agent) in a rabbit lens cell culture. Infect Immun42:1168-75 1983 |
| PubMed ID: 6642663 |
| |
| Reddan JR, Friedman TB, Mostafapour MK, Bondy RL, Sutherland SH, McGee SJ, Goldenberg EM, Donor age influences the growth of rabbit lens epithelial cells in vitro. Vision Res21:11-23 1981 |
| PubMed ID: 7269278 |
| Cumulative PDL at Freeze |
112 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|