AG04762
Endothelial from Aorta, thoracic
Description:
BOS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Endothelial
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Endothelial from Aorta, thoracic
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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60,XY
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Species
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Bos taurus
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Common Name
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cow
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Remarks
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| PDL at Freeze |
46 |
| Passage Frozen |
13 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Holstein breed. The culture was initiated on 4/25/79 using cells released by collagenase digestion from the lumen of a thoracic aorta segment obtained from a fetus of approximately 18 weeks gestational age. The culture was passaged several times in 5% plasma-derived serum to prevent overgrowth by smooth muscle cells. The cell morphology is polygonal. The karyotype is 60,XY; normal diploid male. The culture tested negative for presence of bovine viruses BVD, PI-3, and BHV-1. Same culture at an earlier passage is AG07680B. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Tran QK, Black DJ, Persechini A, Intracellular coupling via limiting calmodulin. J Biol Chem278(27):24247-50 2003 |
| PubMed ID: 12738782 |
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| Gunn J, Holt CM, Francis SE, Shepherd L, Grohmann M, Newman CM, Crossman DC, Cumberland DC, The effect of oligonucleotides to c-myb on vascular smooth muscle cell proliferation and neointima formation after porcine coronary angioplasty. Circ Res80(4):520-31 1997 |
| PubMed ID: 9118483 |
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| Miller DK, Sadowski S, Soderman DD, Kuehl FA Jr, Endothelial cell prostacyclin production induced by activated neutrophils. J Biol Chem260:1006-14 1985 |
| PubMed ID: 3881419 |
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| Thomas KA, Rios-Candelore M, Gimenez-Gallego G, DiSalvo J, Bennett C, Rodkey J, Fitzpatrick S, Pure brain-derived acidic fibroblast growth factor is a potent angiogenic vascular endothelial cell mitogen with sequence homology to interleukin 1. Proc Natl Acad Sci U S A82:6409-13 1985 |
| PubMed ID: 2413439 |
| Cumulative PDL at Freeze |
46 |
| Passage Frozen |
13 |
| Split Ratio |
1:10 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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